Anal Biochem. 1990 Nov 1;190(2):266-70.

The preparation of highly enriched fractions of binucleated rat hepatocytes by centrifugal elutriation and flow cytometry.

Davies R, Cain K, Edwards RE, Snowden RT, Legg RF, Neal GE.

Source

MRC Toxicology Unit, MRC Laboratories, Carshalton, Surrey, United Kingdom.

Abstract

A procedure is described for the isolation of highly enriched fractions of binucleated hepatocytes from rat liver. Liver cells isolated by EGTA and collagenase perfusion were initially subjected to centrifugal elutriation and second to flow cytometry coupled with Hoechst 33342 staining. The elutriation step yielded hepatocyte fractions which contained almost entirely mononuclear diploid cells and fractions enriched in binucleate hepatocytes. The fractions with the highest proportion of binucleated hepatocytes contained between 50 and 56% of these cells. Subsequent flow cytometric cell sorting yielded fractions which contained greater than 80% binucleated cells. These cells were viable in culture as demonstrated by the immunohistochemical detection of bromodeoxyuridine incorporation.

PMID:: 1705398; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

BROMODEOXYURIDINE - HSDB

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Isolation of preneoplastic rat liver cells by centrifugal elutriation and binding to asialofetuin. [Cancer Res. 1984]
Isolation of preneoplastic rat liver cells by centrifugal elutriation and binding to asialofetuin.
Evarts RP, Marsden E, Hanna P, Wirth PJ, Thorgeirsson SS. Cancer Res. 1984 Dec; 44(12 Pt 1):5718-24.
Heterogeneous distribution of drug metabolism in elutriated rat hepatocytes. [Biochem Pharmacol. 1985]
Heterogeneous distribution of drug metabolism in elutriated rat hepatocytes.
Willson RA, Liem HH, Miyai K, Muller-Eberhard U. Biochem Pharmacol. 1985 May 1; 34(9):1463-70.
Hepatocyte ploidy in normal young rat. [Comp Biochem Physiol A Mol Int...]
Hepatocyte ploidy in normal young rat.
Gandillet A, Alexandre E, Holl V, Royer C, Bischoff P, Cinqualbre J, Wolf P, Jaeck D, Richert L. Comp Biochem Physiol A Mol Integr Physiol. 2003 Mar; 134(3):665-73.
Centrifugal elutriation of hepatocytes from 2-acetylaminofluorene-treated rats and their characterization by flow cytometry. [Carcinogenesis. 1989]
Centrifugal elutriation of hepatocytes from 2-acetylaminofluorene-treated rats and their characterization by flow cytometry.
Klose U, Thierau D, Greim H, Schwarz LR. Carcinogenesis. 1989 Mar; 10(3):553-6.
Review Isolation and analyses of enriched populations of male mouse germ cells by sedimentation velocity: the centrifugal elutriation. [Methods Mol Biol. 2009]
Review Isolation and analyses of enriched populations of male mouse germ cells by sedimentation velocity: the centrifugal elutriation.
Barchi M, Geremia R, Magliozzi R, Bianchi E. Methods Mol Biol. 2009; 558:299-321.

See reviews... See all...

Cited by 1 PubMed Central article

Involvement of HTLV-I Tax and CREB in aneuploidy: a bioinformatics approach. [Retrovirology. 2006]
Involvement of HTLV-I Tax and CREB in aneuploidy: a bioinformatics approach.
de la Fuente C, Gupta MV, Klase Z, Strouss K, Cahan P, McCaffery T, Galante A, Soteropoulos P, Pumfery A, Fujii M, et al. Retrovirology. 2006 Jul 5; 3:43. Epub 2006 Jul 5.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The preparation of highly enriched fractions of binucleated rat hepatocytes by c...
The preparation of highly enriched fractions of binucleated rat hepatocytes by centrifugal elutriation and flow cytometry.
Anal Biochem. 1990 Nov 1 ;190(2):266-70.

PubMed
Global and regional distribution of HIV-1 genetic subtypes and recombinants in 2...
Global and regional distribution of HIV-1 genetic subtypes and recombinants in 2004.
AIDS. 2006 Oct 24 ;20(16):W13-23.

PubMed
Transforming growth factor beta suppresses human immunodeficiency virus expressi...
Transforming growth factor beta suppresses human immunodeficiency virus expression and replication in infected cells of the monocyte/macrophage lineage.
J Exp Med. 1991 Mar 1 ;173(3):589-97.

PubMed
Human T-cell leukemia virus type 1 Tax protein down-regulates pre-T-cell recepto...
Human T-cell leukemia virus type 1 Tax protein down-regulates pre-T-cell receptor alpha gene transcription in human immature thymocytes.
J Virol. 2007 Jan ;81(1):301-8. Epub 2006 Oct 18 .

PubMed
Suppression of human immunodeficiency virus expression in chronically infected m...
Suppression of human immunodeficiency virus expression in chronically infected monocytic cells by glutathione, glutathione ester, and N-acetylcysteine.
Proc Natl Acad Sci U S A. 1991 Feb 1 ;88(3):986-90.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: