J Exp Med. 1990 Nov 1;172(5):1377-90.

Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.

Source

Division of Immunology, Medical Biology Institute, La Jolla, California 92037.

Abstract

Much of T and B lymphocyte receptor diversity derives from the addition of nontemplated N regions at the junctions of receptor gene elements, although fetal T cells expressing gamma/delta receptors lack N regions. I have sequenced immunoglobulin H chain variable regions of PCR-amplified DNA and cDNA from fetal and newborn mouse liver and spleen cells. These sequences showed an absence of N regions. Only 1/87 DNA sequences and 17/146 RNA sequences contained N regions, in striking contrast to adult Ig sequences. These data show that N region insertion is a developmentally regulated process in B cells as well as in T cells, and demonstrate that receptor diversity in neonatal B cells is limited by the absence of N regions as well as by biased usage of Vh genes.

PMID:: 1700054; [PubMed - indexed for MEDLINE]
PMCID:: PMC2188672

Free PMC Article

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Junctional sequences of fetal T cell receptor beta chains have few N regions. [J Exp Med. 1991]
Junctional sequences of fetal T cell receptor beta chains have few N regions.
Feeney AJ. J Exp Med. 1991 Jul 1; 174(1):115-24.
Predominance of the prototypic T15 anti-phosphorylcholine junctional sequence in neonatal pre-B cells. [J Immunol. 1991]
Predominance of the prototypic T15 anti-phosphorylcholine junctional sequence in neonatal pre-B cells.
Feeney AJ. J Immunol. 1991 Dec 15; 147(12):4343-50.
Junctional diversity in the absence of N regions. Neonatal T cell receptor beta chain junctional sequences are more heterogeneous than neonatal T cell receptor gamma delta or IgH junctions. [J Immunol. 1993]
Junctional diversity in the absence of N regions. Neonatal T cell receptor beta chain junctional sequences are more heterogeneous than neonatal T cell receptor gamma delta or IgH junctions.
Feeney AJ. J Immunol. 1993 Sep 15; 151(6):3094-9.
Review Comparison of junctional diversity in the neonatal and adult immunoglobulin repertoires. [Int Rev Immunol. 1992]
Review Comparison of junctional diversity in the neonatal and adult immunoglobulin repertoires.
Feeney AJ. Int Rev Immunol. 1992; 8(2-3):113-22.
Review Influence of the V(D)J recombination mechanism on the formation of the primary T and B cell repertoires. [Semin Immunol. 1994]
Review Influence of the V(D)J recombination mechanism on the formation of the primary T and B cell repertoires.
Feeney AJ, Victor KD, Vu K, Nadel B, Chukwuocha RU. Semin Immunol. 1994 Jun; 6(3):155-63.

See reviews... See all...

Cited by 73 PubMed Central articles

Absence of N addition facilitates B cell development, but impairs immune responses. [Immunogenetics. 2011]
Absence of N addition facilitates B cell development, but impairs immune responses.
Schelonka RL, Ivanov II, Vale AM, Dimmitt RA, Khaled M, Schroeder HW Jr. Immunogenetics. 2011 Sep; 63(9):599-609. Epub 2011 Jun 10.
Alternative mechanisms of receptor editing in autoreactive B cells. [Proc Natl Acad Sci U S A. 2011]
Alternative mechanisms of receptor editing in autoreactive B cells.
Kalinina O, Doyle-Cooper CM, Miksanek J, Meng W, Prak EL, Weigert MG. Proc Natl Acad Sci U S A. 2011 Apr 26; 108(17):7125-30. Epub 2011 Apr 6.
Determinism and stochasticity during maturation of the zebrafish antibody repertoire. [Proc Natl Acad Sci U S A. 2011]
Determinism and stochasticity during maturation of the zebrafish antibody repertoire.
Jiang N, Weinstein JA, Penland L, White RA 3rd, Fisher DS, Quake SR. Proc Natl Acad Sci U S A. 2011 Mar 29; 108(13):5348-53. Epub 2011 Mar 10.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional se...
Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.
J Exp Med. 1990 Nov 1 ;172(5):1377-90.

PubMed
Systematic reviews of qualitative evidence: what are the experiences of women wi...
Systematic reviews of qualitative evidence: what are the experiences of women with endometriosis?
J Obstet Gynaecol. 2006 Aug ;26(6):501-6.

PubMed
Comparison of cisplatin and carboplatin cytotoxicity in human ovarian cancer cel...
Comparison of cisplatin and carboplatin cytotoxicity in human ovarian cancer cell lines using the MTT assay.
Gynecol Oncol. 1990 Nov ;39(2):119-22.

PubMed
AMP-activated protein kinase--the fat controller of the energy railroad.
AMP-activated protein kinase--the fat controller of the energy railroad.
Can J Physiol Pharmacol. 2006 Jul ;84(7):655-65.

PubMed
Primary structure of cAMP-gated channel from bovine olfactory epithelium.
Primary structure of cAMP-gated channel from bovine olfactory epithelium.
FEBS Lett. 1990 Sep 17 ;270(1-2):24-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 73 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information