Nat Methods. 2006 Sep;3(9):715-9.

Genome-scale loss-of-function screening with a lentiviral RNAi library.

Root DE, Hacohen N, Hahn WC, Lander ES, Sabatini DM.

Source

Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. droot@broad.mit.edu

Abstract

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

PMID:: 16929317; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

RNA, Small Interfering

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. [Cell. 2006]
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen.
Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, et al. Cell. 2006 Mar 24; 124(6):1283-98.
Review Genome-wide screening for gene function using RNAi in mammalian cells. [Immunol Cell Biol. 2005]
Review Genome-wide screening for gene function using RNAi in mammalian cells.
Cullen LM, Arndt GM. Immunol Cell Biol. 2005 Jun; 83(3):217-23.
MISSION LentiPlex pooled shRNA library screening in mammalian cells. [J Vis Exp. 2011]
MISSION LentiPlex pooled shRNA library screening in mammalian cells.
Coussens MJ, Corman C, Fischer AL, Sago J, Swarthout J. J Vis Exp. 2011 Dec 21; (58). Epub 2011 Dec 21.
Review shRNA libraries and their use in cancer genetics. [Nat Methods. 2006]
Review shRNA libraries and their use in cancer genetics.
Bernards R, Brummelkamp TR, Beijersbergen RL. Nat Methods. 2006 Sep; 3(9):701-6.
A resource for large-scale RNA-interference-based screens in mammals. [Nature. 2004]
A resource for large-scale RNA-interference-based screens in mammals.
Paddison PJ, Silva JM, Conklin DS, Schlabach M, Li M, Aruleba S, Balija V, O'Shaughnessy A, Gnoj L, Scobie K, et al. Nature. 2004 Mar 25; 428(6981):427-31.

See reviews... See all...

Cited by 48 PubMed Central articles

A common role for various human truncated adenomatous polyposis coli isoforms in the control of beta-catenin activity and cell proliferation. [PLoS One. 2012]
A common role for various human truncated adenomatous polyposis coli isoforms in the control of beta-catenin activity and cell proliferation.
Chandra SH, Wacker I, Appelt UK, Behrens J, Schneikert J. PLoS One. 2012; 7(4):e34479. Epub 2012 Apr 3.
Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses. [Retrovirology. 2012]
Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses.
Simonis N, Rual JF, Lemmens I, Boxus M, Hirozane-Kishikawa T, Gatot JS, Dricot A, Hao T, Vertommen D, Legros S, et al. Retrovirology. 2012 Mar 29; 9:26. Epub 2012 Mar 29.
Inhibition of HIV-1 replication with stable RNAi-mediated knockdown of autophagy factors. [Virol J. 2012]
Inhibition of HIV-1 replication with stable RNAi-mediated knockdown of autophagy factors.
Eekels JJ, Sagnier S, Geerts D, Jeeninga RE, Biard-Piechaczyk M, Berkhout B. Virol J. 2012 Mar 16; 9(1):69. Epub 2012 Mar 16.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Genome-scale loss-of-function screening with a lentiviral RNAi library.
Genome-scale loss-of-function screening with a lentiviral RNAi library.
Nat Methods. 2006 Sep ;3(9):715-9.

PubMed
The intron of the yeast actin gene contains the promoter for an antisense RNA.
The intron of the yeast actin gene contains the promoter for an antisense RNA.
Curr Genet. 1990 Mar ;17(3):269-73.

PubMed
Early events of Bacillus anthracis germination identified by time-course quantit...
Early events of Bacillus anthracis germination identified by time-course quantitative proteomics.
Proteomics. 2006 Oct ;6(19):5199-211.

PubMed
Identification of Francisella species and discrimination of type A and type B st...
Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis.
Appl Environ Microbiol. 1990 Apr ;56(4):949-55.

PubMed
Extensive genomic plasticity in Pseudomonas aeruginosa revealed by identificatio...
Extensive genomic plasticity in Pseudomonas aeruginosa revealed by identification and distribution studies of novel genes among clinical isolates.
Infect Immun. 2006 Sep ;74(9):5272-83.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: