J Clin Pathol. 1990 Mar;43(3):213-9.

Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.

Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H.

Source

Department of Pathology, Klinikum Steglitz, Free University Berlin, West Germany.

Abstract

A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinomas and all non-neoplastic epithelial cells, except hepatocytes, parietal cells, and apical cell layers in squamous epithelia, homogeneously expressed Ber-EP4 antigen. As Ber-EP4 does not detect any normal or neoplastic non-epithelial cells, this antibody might prove valuable for the differentiation of the following (i) non-epithelial tumours from undifferentiated carcinomas; (ii) hepatocytes from bile duct cells in certain liver diseases; (iii) mesothelial cells from carcinoma cells in lung biopsy specimens; and (iv) reactive mesothelial cells from carcinoma cells in smears of serous effusions.

PMID:: 1692040; [PubMed - indexed for MEDLINE]
PMCID:: PMC502333

Free PMC Article

LinkOut - more resources

Full Text Sources

Medical

Research Materials

anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) antibody - antibodies-online

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Immunocytochemical staining of serous effusions with the monoclonal antibody Ber-EP4. [Cytopathology. 1992]
Immunocytochemical staining of serous effusions with the monoclonal antibody Ber-EP4.
De Angelis M, Buley ID, Heryet A, Gray W. Cytopathology. 1992; 3(2):111-7.
Ber-EP4 and anti-calretinin antibodies: a useful combination for differential diagnosis of various histological types of ovarian cancer cells and mesothelial cells. [Tohoku J Exp Med. 2005]
Ber-EP4 and anti-calretinin antibodies: a useful combination for differential diagnosis of various histological types of ovarian cancer cells and mesothelial cells.
Okamoto S, Ito K, Sasano H, Moriya T, Niikura H, Terada Y, Sato S, Okamura K, Yaegashi N. Tohoku J Exp Med. 2005 May; 206(1):31-40.
Monoclonal antibody Ber-EP4: its use in the differential diagnosis of malignant mesothelioma and carcinoma in cell blocks of malignant effusions and FNA specimens. [Diagn Cytopathol. 1994]
Monoclonal antibody Ber-EP4: its use in the differential diagnosis of malignant mesothelioma and carcinoma in cell blocks of malignant effusions and FNA specimens.
Maguire B, Whitaker D, Carrello S, Spagnolo D. Diagn Cytopathol. 1994; 10(2):130-4.
Review Value of the Ber-EP4 antibody in differentiating epithelial pleural mesothelioma from adenocarcinoma. The M.D. Anderson experience and a critical review of the literature. [Am J Clin Pathol. 1998]
Review Value of the Ber-EP4 antibody in differentiating epithelial pleural mesothelioma from adenocarcinoma. The M.D. Anderson experience and a critical review of the literature.
Ordóñez NG. Am J Clin Pathol. 1998 Jan; 109(1):85-9.
Review Applications of monoclonal antibodies in clinical cytology as exemplified by studies with monoclonal antibody B72.3. The George N. Papanicolaou award lecture. [Acta Cytol. 1987]
Review Applications of monoclonal antibodies in clinical cytology as exemplified by studies with monoclonal antibody B72.3. The George N. Papanicolaou award lecture.
Johnston WW. Acta Cytol. 1987 Sep-Oct; 31(5):537-56.

See reviews... See all...

Cited by 23 PubMed Central articles

Isolation and characterization of intestinal epithelial cells from normal and SIV-infected rhesus macaques. [PLoS One. 2012]
Isolation and characterization of intestinal epithelial cells from normal and SIV-infected rhesus macaques.
Pan D, Das A, Liu D, Veazey RS, Pahar B. PLoS One. 2012; 7(1):e30247. Epub 2012 Jan 26.
Clinical significance of CDX2-positive circulating tumour cells in colorectal cancer patients. [Br J Cancer. 2011]
Clinical significance of CDX2-positive circulating tumour cells in colorectal cancer patients.
Wong SC, Ng SS, Cheung MT, Luk LY, Chan CM, Cheung AH, Lee VH, Lai PB, Ma BB, Hui EP, et al. Br J Cancer. 2011 Mar 15; 104(6):1000-6. Epub 2011 Mar 1.
Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies. [BMC Biotechnol. 2010]
Heterogeneous detection of circulating tumor cells in patients with colorectal cancer by immunomagnetic enrichment using different EpCAM-specific antibodies.
Antolovic D, Galindo L, Carstens A, Rahbari N, Büchler MW, Weitz J, Koch M. BMC Biotechnol. 2010 Apr 28; 10:35. Epub 2010 Apr 28.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.
Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.
J Clin Pathol. 1990 Mar ;43(3):213-9.

PubMed
Comparison of gene expression profiles in core biopsies and corresponding surgic...
Comparison of gene expression profiles in core biopsies and corresponding surgical breast cancer samples.
Breast Cancer Res. 2006 ;8(4):R51.

PubMed
Pattern of interleukin 6 gene expression in vivo suggests a role for this cytoki...
Pattern of interleukin 6 gene expression in vivo suggests a role for this cytokine in angiogenesis.
Proc Natl Acad Sci U S A. 1990 Apr ;87(8):3092-6.

PubMed
Effect of bortezomib on human neuroblastoma cell growth, apoptosis, and angiogen...
Effect of bortezomib on human neuroblastoma cell growth, apoptosis, and angiogenesis.
J Natl Cancer Inst. 2006 Aug 16 ;98(16):1142-57.

PubMed
Keratins as markers that distinguish normal and tumor-derived mammary epithelial...
Keratins as markers that distinguish normal and tumor-derived mammary epithelial cells.
Proc Natl Acad Sci U S A. 1990 Mar ;87(6):2319-23.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Research Materials

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 23 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information