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    Development. 2006 Sep;133(18):3563-73. Epub 2006 Aug 16.

    Small molecule-induced ablation and subsequent regeneration of larval zebrafish melanocytes.

    Yang CT, Johnson SL.

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    Department of Genetics, Washington University School of Medicine, 4566 Scott Avenue, Saint Louis, MO 63110, USA.

    Erratum in

    • Development. 2006 Nov;133(22):4607.

    Abstract

    We developed a method to efficiently ablate a single cell type, the zebrafish melanocyte, and study the mechanisms of its regeneration. We found that a small molecule, (2-morpholinobutyl)-4-thiophenol (MoTP), specifically ablates zebrafish larval melanocytes or melanoblasts, and that this melanocytotoxicity is dependent on tyrosinase activity, which presumably converts MoTP to cytotoxic quinone species. Following melanocyte ablation by MoTP treatment, we demonstrate by BrdU incorporation experiments that regenerated melanocytes are derived from the division of otherwise quiescent melanocyte precursors or stem cells. We further show that larval melanocyte regeneration requires the kit receptor tyrosine kinase. Our results suggest that a small number of melanocyte precursors or stem cells unevenly distributed in larvae are drawn upon to reconstitute the larval melanocyte population following melanocyte ablation by MoTP.

    PMID:
    16914496
    [PubMed - indexed for MEDLINE]
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