Biotechnol Bioeng. 2007 Feb 15;96(3):615-21.

Propagation of fluorescent viruses in growing plaques.

Alvarez LJ, Thomen P, Makushok T, Chatenay D.

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Laboratoire de Physique Statistique, Ecole Normale Supérieure, Paris, France.

Abstract

To study virus propagation, we have developed a method by which the propagation of the Lambda bacteriophage can be observed and quantified. This is done by creating a fusion protein of the capsid protein gpD and the enhanced yellow fluorescent protein (EYFP). We show that this fusion allows capsid formation and that the modified viruses propagate on a surface covered with host bacteria thus forming fluorescent plaques. The intensity of fluorescence in a growing plaque determines the distribution of phages. This provides a new tool to study the propagation of infection at the microscopic level.

PMID:: 16900526; [PubMed - indexed for MEDLINE]

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