Retrovirology. 2006 May 25;3:29.

Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates.

Kim FJ, Lavanya M, Gessain A, Gallego S, Battini JL, Sitbon M, Courgnaud V.

Source

Institut de Génétique Moléculaire de Montpellier (IGMM), 1919 Rte de Mende, F-34293 Montpellier Cedex 5, France. kimf@mskcc.org

Abstract

Four primate (PTLV), human (HTLV) and simian (STLV) T-cell leukemia virus types, have been characterized thus far, with evidence of a simian zoonotic origin for HTLV-1, HTLV-2 and HTLV-3 in Africa. The PTLV envelope glycoprotein surface component (SUgp46) comprises a receptor-binding domain (RBD) that alternates hypervariable and highly conserved sequences. To further delineate highly conserved motifs in PTLV RBDs, we investigated the intrahost variability of HTLV-1 and STLV-1 by generating and sequencing libraries of DNA fragments amplified within the RBD of the SUgp46 env gene. Using new and highly cross-reactive env primer pairs, we observed the presence of Env quasispecies in HTLV-1 infected individuals and STLV-1 naturally infected macaques, irrespective of the clinical status. These intrahost variants helped us to define highly conserved residues and motifs in the RBD. The new highly sensitive env PCR described here appears suitable for the screening of all known variants of the different PTLV types and should, therefore, be useful for the analysis of seroindeterminate samples.

PMID:: 16725042; [PubMed - indexed for MEDLINE]
PMCID: PMC1481548

Free PMC Article

Images from this publication.See all images (1) Free text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Viral Envelope Proteins

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Related citations

Simian T-cell leukemia virus (STLV) infection in wild primate populations in Cameroon: evidence for dual STLV type 1 and type 3 infection in agile mangabeys (Cercocebus agilis). [J Virol. 2004]
Simian T-cell leukemia virus (STLV) infection in wild primate populations in Cameroon: evidence for dual STLV type 1 and type 3 infection in agile mangabeys (Cercocebus agilis).
Courgnaud V, Van Dooren S, Liegeois F, Pourrut X, Abela B, Loul S, Mpoudi-Ngole E, Vandamme A, Delaporte E, Peeters M. J Virol. 2004 May; 78(9):4700-9.
Use of a generic polymerase chain reaction assay detecting human T-lymphotropic virus (HTLV) types I, II and divergent simian strains in the evaluation of individuals with indeterminate HTLV serology. [J Med Virol. 1997]
Use of a generic polymerase chain reaction assay detecting human T-lymphotropic virus (HTLV) types I, II and divergent simian strains in the evaluation of individuals with indeterminate HTLV serology.
Vandamme AM, Van Laethem K, Liu HF, Van Brussel M, Delaporte E, de Castro Costa CM, Fleischer C, Taylor G, Bertazzoni U, Desmyter J, et al. J Med Virol. 1997 May; 52(1):1-7.
Isolation and characterization of a new simian T-cell leukemia virus type 1 from naturally infected celebes macaques (Macaca tonkeana): complete nucleotide sequence and phylogenetic relationship with the Australo-Melanesian human T-cell leukemia virus type 1. [J Virol. 1995]
Isolation and characterization of a new simian T-cell leukemia virus type 1 from naturally infected celebes macaques (Macaca tonkeana): complete nucleotide sequence and phylogenetic relationship with the Australo-Melanesian human T-cell leukemia virus type 1.
Ibrahim F, de Thé G, Gessain A. J Virol. 1995 Nov; 69(11):6980-93.
Review [Epidemiology, origin and genetic diversity of HTLV-1 retrovirus and STLV-1 simian affiliated retrovirus]. [Bull Soc Pathol Exot. 2000]
Review [Epidemiology, origin and genetic diversity of HTLV-1 retrovirus and STLV-1 simian affiliated retrovirus].
Gessain A, Mahieux R. Bull Soc Pathol Exot. 2000 Jul; 93(3):163-71.
Review Implications of the evolution pattern of human T-cell leukemia retroviruses on their pathogenic virulence (Review). [Int J Mol Med. 2004]
Review Implications of the evolution pattern of human T-cell leukemia retroviruses on their pathogenic virulence (Review).
Azran I, Schavinsky-Khrapunsky Y, Priel E, Huleihel M, Aboud M. Int J Mol Med. 2004 Nov; 14(5):909-15.

See reviews... See all...

Cited by 2 PubMed Central articles

Molecular aspects of HTLV-1 entry: functional domains of the HTLV-1 surface subunit (SU) and their relationships to the entry receptors. [Viruses. 2011]
Molecular aspects of HTLV-1 entry: functional domains of the HTLV-1 surface subunit (SU) and their relationships to the entry receptors.
Jones KS, Lambert S, Bouttier M, Bénit L, Ruscetti FW, Hermine O, Pique C. Viruses. 2011 Jun; 3(6):794-810. Epub 2011 Jun 15.
No evidence for XMRV association in pediatric idiopathic diseases in France. [Retrovirology. 2010]
No evidence for XMRV association in pediatric idiopathic diseases in France.
Jeziorski E, Foulongne V, Ludwig C, Louhaem D, Chiocchia G, Segondy M, Rodière M, Sitbon M, Courgnaud V. Retrovirology. 2010 Aug 2; 7:63. Epub 2010 Aug 2.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
PopSet
Sets of sequences from population and evolutionary genetic studies in the PopSet database reported in the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and...
Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates.
Retrovirology. 2006 May 25 ;3:29.

PubMed
The formation of cysteine-linked dimers of BST-2/tetherin is important for inhib...
The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu.
Retrovirology. 2009 Sep 8 ;6:80.

PubMed
Inhibition of HIV-1 replication by small interfering RNAs directed against gliom...
Inhibition of HIV-1 replication by small interfering RNAs directed against glioma pathogenesis related protein (GliPR) expression.
Retrovirology. 2010 Mar 31 ;7:26.

PubMed
On the steps of cell-to-cell HIV transmission between CD4 T cells.
On the steps of cell-to-cell HIV transmission between CD4 T cells.
Retrovirology. 2009 Oct 13 ;6:89.

PubMed
A single amino acid substitution of the human immunodeficiency virus type 1 caps...
A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5 alpha.
Retrovirology. 2010 Jul 7 ;7:58.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: