Lancet. 1991 Mar 16;337(8742):640-2.

HLA class I typing by PCR: HLA-B27 and an African B27 subtype.

Hill AV, Allsopp CE, Kwiatkowski D, Anstey NM, Greenwood BM, McMichael AJ.

Source

Molecular Immunology Group, University of Oxford, John Radcliffe Hospital.

Abstract

We describe a rapid method of HLA class I typing using the polymerase chain reaction and oligonucleotide hybridisation that eliminates the requirements for viable lymphocytes and allows subtypes to be defined. We have used this to demonstrate that the predominant subtype of HLA-B27 in the Gambia, West Africa, is HLA-B*2703, which is very rare or absent in other racial groups. This subtype differs from the common Caucasian HLA-B27 subtypes in its recognition by cytotoxic T cells. We propose that HLA*B-2703, unlike other HLA-B27 subtypes, may not be associated with ankylosing spondylitis, thus accounting in part for the rarity of this condition in black populations.

Comment in

Lancet. 1991 Apr 13;337(8746):927.

PMID:: 1671992; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

HLA-B27 Antigen

Grant Support

Wellcome Trust/United Kingdom

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