Science. 2006 May 19;312(5776):1044-6. Epub 2006 Apr 27.

Emergent properties of reduced-genome Escherichia coli.

Pósfai G, Plunkett G 3rd, Fehér T, Frisch D, Keil GM, Umenhoffer K, Kolisnychenko V, Stahl B, Sharma SS, de Arruda M, Burland V, Harcum SW, Blattner FR.

Source

Institute of Biochemistry, Biological Research Center, H-6726 Szeged, Hungary. posfaigy@nucleus.szbk.u-szeged.hu

Abstract

With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties.

PMID:: 16645050; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

GM35682/GM/NIGMS NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12. [Methods Mol Biol. 2008]
The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12.
Baba T, Huan HC, Datsenko K, Wanner BL, Mori H. Methods Mol Biol. 2008; 416:183-94.
Engineering a reduced Escherichia coli genome. [Genome Res. 2002]
Engineering a reduced Escherichia coli genome.
Kolisnychenko V, Plunkett G 3rd, Herring CD, Fehér T, Pósfai J, Blattner FR, Pósfai G. Genome Res. 2002 Apr; 12(4):640-7.
Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes. [J Mol Biol. 2009]
Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes.
Studier FW, Daegelen P, Lenski RE, Maslov S, Kim JF. J Mol Biol. 2009 Dec 11; 394(4):653-80. Epub 2009 Sep 15.
Review IS elements as constituents of bacterial genomes. [Res Microbiol. 1999]
Review IS elements as constituents of bacterial genomes.
Mahillon J, Léonard C, Chandler M. Res Microbiol. 1999 Nov-Dec; 150(9-10):675-87.
Review Insertion sequences in prokaryotic genomes. [Curr Opin Microbiol. 2006]
Review Insertion sequences in prokaryotic genomes.
Siguier P, Filée J, Chandler M. Curr Opin Microbiol. 2006 Oct; 9(5):526-31. Epub 2006 Aug 28.

See reviews... See all...

Cited by 48 PubMed Central articles

Identification and Characterization of Microcin S, a New Antibacterial Peptide Produced by Probiotic Escherichia coli G3/10. [PLoS One. 2012]
Identification and Characterization of Microcin S, a New Antibacterial Peptide Produced by Probiotic Escherichia coli G3/10.
Zschüttig A, Zimmermann K, Blom J, Goesmann A, Pöhlmann C, Gunzer F. PLoS One. 2012; 7(3):e33351. Epub 2012 Mar 30.
Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs. [Microb Cell Fact. 2012]
Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs.
Csörgo B, Fehér T, Tímár E, Blattner FR, Pósfai G. Microb Cell Fact. 2012 Jan 20; 11:11. Epub 2012 Jan 20.
Genome Size and Species Diversification. [Evol Biol. 2010]
Genome Size and Species Diversification.
Kraaijeveld K. Evol Biol. 2010 Dec; 37(4):227-233. Epub 2010 Sep 17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Emergent properties of reduced-genome Escherichia coli.
Emergent properties of reduced-genome Escherichia coli.
Science. 2006 May 19 ;312(5776):1044-6. Epub 2006 Apr 27 .

PubMed
Modulation of insulin secretion by diazepam binding inhibitor and its processing...
Modulation of insulin secretion by diazepam binding inhibitor and its processing products.
Neuropharmacology. 1991 Dec ;30(12B):1399-403.

PubMed
Relationships in Alzheimer's disease between the extent of Abeta deposition in c...
Relationships in Alzheimer's disease between the extent of Abeta deposition in cerebral blood vessel walls, as cerebral amyloid angiopathy, and the amount of cerebrovascular smooth muscle cells and collagen.
Neuropathol Appl Neurobiol. 2006 Jun ;32(3):332-40.

PubMed
Hydroxyl radical footprinting.
Hydroxyl radical footprinting.
Methods Enzymol. 1991 ;208:380-413.

PubMed
Outcomes of clinical trial: tinnitus masking versus tinnitus retraining therapy.
Outcomes of clinical trial: tinnitus masking versus tinnitus retraining therapy.
J Am Acad Audiol. 2006 Feb ;17(2):104-32.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: