Nucleic Acids Res. 1991 Dec 25;19(24):6691-8.

Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein.

Vink C, Yeheskiely E, van der Marel GA, van Boom JH, Plasterk RH.

Source

Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam.

Abstract

Before integration of the human immunodeficiency virus (HIV) DNA, two nucleotides are removed from the 3' ends of the viral DNA by the integrase (IN) protein. We studied the chemistry of this reaction, and found that IN mediates site-specific hydrolysis of a phosphodiester bond, resulting in release of a dinucleotide. A class of alcohols (including glycerol, 1,2-propanediol, but not 1,3-propanediol) can also act as nucleophile in this reaction, and likewise the alcoholic amino acids L-serine and L-threonine can be covalently linked to the dinucleotide. No evidence was found for a covalent linkage between the IN protein and this dinucleotide, suggesting that IN directs a single nucleophilic attack of water at the specific phosphodiester bond.

PMID:: 1662361; [PubMed - indexed for MEDLINE]
PMCID:: PMC329296

Free PMC Article

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Identification of amino acids in HIV-2 integrase involved in site-specific hydrolysis and alcoholysis of viral DNA termini. [Nucleic Acids Res. 1993]
Identification of amino acids in HIV-2 integrase involved in site-specific hydrolysis and alcoholysis of viral DNA termini.
van Gent DC, Oude Groeneger AA, Plasterk RH. Nucleic Acids Res. 1993 Jul 25; 21(15):3373-7.
Juxtaposition of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or murine leukemia virus integrase. [J Virol. 1994]
Juxtaposition of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or murine leukemia virus integrase.
Chow SA, Brown PO. J Virol. 1994 Dec; 68(12):7869-78.
DNA binding properties of the integrase proteins of human immunodeficiency viruses types 1 and 2. [Nucleic Acids Res. 1991]
DNA binding properties of the integrase proteins of human immunodeficiency viruses types 1 and 2.
van Gent DC, Elgersma Y, Bolk MW, Vink C, Plasterk RH. Nucleic Acids Res. 1991 Jul 25; 19(14):3821-7.
Review HIV integration. Ini1 for integration? [Curr Biol. 1995]
Review HIV integration. Ini1 for integration?
Miller MD, Bushman FD. Curr Biol. 1995 Apr 1; 5(4):368-70.
Review Retroviral DNA integration. [Microbiol Mol Biol Rev. 1999]
Review Retroviral DNA integration.
Hindmarsh P, Leis J. Microbiol Mol Biol Rev. 1999 Dec; 63(4):836-43, table of contents.

See reviews... See all...

Cited by 54 PubMed Central articles

Interactions of host proteins with the murine leukemia virus integrase. [Viruses. 2010]
Interactions of host proteins with the murine leukemia virus integrase.
Studamire B, Goff SP. Viruses. 2010 May 5; 2(5):1110-45.
Deciphering the code for retroviral integration target site selection. [PLoS Comput Biol. 2010]
Deciphering the code for retroviral integration target site selection.
Santoni FA, Hartley O, Luban J. PLoS Comput Biol. 2010 Nov 24; 6(11):e1001008. Epub 2010 Nov 24.
In vitro HIV-1 selective integration into the target sequence and decoy-effect of the modified sequence. [PLoS One. 2010]
In vitro HIV-1 selective integration into the target sequence and decoy-effect of the modified sequence.
Tsuruyama T, Nakai T, Hiratsuka T, Jin G, Nakamura T, Yoshikawa K. PLoS One. 2010 Nov 4; 5(11):e13841. Epub 2010 Nov 4.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA ter...
Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein.
Nucleic Acids Res. 1991 Dec 25 ;19(24):6691-8.

PubMed
Global epidemiology of HIV.
Global epidemiology of HIV.
J Med Virol. 2006 ;78 Suppl 1:S7-S12.

PubMed
An activating mutation in the murine erythropoietin receptor induces erythroleuk...
An activating mutation in the murine erythropoietin receptor induces erythroleukemia in mice: a cytokine receptor superfamily oncogene.
Cell. 1991 Dec 20 ;67(6):1089-102.

PubMed
Antiviral drug resistance.
Antiviral drug resistance.
Antiviral Res. 2006 Sep ;71(2-3):117-21. Epub 2006 Mar 30 .

PubMed
Efficient selection for high-expression transfectants with a novel eukaryotic ve...
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
Gene. 1991 Dec 15 ;108(2):193-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 54 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information