Abstract
A number of plasmidless strains were obtained by curing the 90 kilobase pair (kb) virulence plasmid from six strains, C5, TML, W118, SR11, LT2 and Fisher, of Salmonella serovar Typhimurium. A number of transposon (Tn5) tagged 90 kb plasmids, also derived from these Typhimurium strains, were then transferred back into these plasmidless strains. Plasmid-cured strains, reconstituted strains, and the parental strains were tested for their virulence in BALB/c mice. There were two groups of Typhimurium strains: one required the 90 kb plasmid to express high virulence (LD50 less than 50 bacteria), and the other, regardless of the presence or absence of the 90 kb plasmid, maintained the same level of virulence at LD50 = 10 to 7 x 10(5) bacteria. Among the plasmidless strains, there were strains with a virulence level as low as LD50 = 10(7) bacteria, which was unaffected by the presence of the 90 kb plasmid.