Virology. 1992 Aug;189(2):725-33.

Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts.

Source

Department of Microbiology, Oregon State University, Corvallis 97331-3804.

Abstract

Transgenic tobacco plants which express untranslatable sense or antisense forms of the tobacco etch virus potyvirus (TEV) coat protein (CP) gene sequence have been generated. One of seven transgenic plant lines expressing a CP gene antisense transcript showed an attenuation of symptoms when inoculated with TEV. Three of ten transgenic plant lines expressing untranslatable sense transcripts did not develop symptoms when inoculated with TEV. These lines were resistant to either aphid or mechanically transmitted TEV. In contrast to CP-mediated resistance reported for other viruses, resistance was (1) mediated by an RNA molecule; (2) TEV-specific (i.e., "broad-spectrum resistance" was not observed); (3) independent of inoculum levels; (4) not dependent on plant size and; (5) due to decreased levels of virus replication. Protoplast experiments were used to demonstrate that resistant plant lines did not support the production of virus protein and progeny virus at wild-type levels.

PMID:: 1641986; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH Terms

Substances

RNA, Viral

LinkOut - more resources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Analysis of transgenic tobacco plants expressing a truncated form of a potyvirus coat protein nucleotide sequence. [Plant Mol Biol. 1994]
Analysis of transgenic tobacco plants expressing a truncated form of a potyvirus coat protein nucleotide sequence.
Silva-Rosales L, Lindbo JA, Dougherty WG. Plant Mol Biol. 1994 Mar; 24(6):929-39.
Pathogen-derived resistance to a potyvirus: immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. [Mol Plant Microbe Interact. 1992]
Pathogen-derived resistance to a potyvirus: immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence.
Lindbo JA, Dougherty WG. Mol Plant Microbe Interact. 1992 Mar-Apr; 5(2):144-53.
[The viral resistance of transgenic tobacco plants containing untranslatable PVYN coat protein gene]. [Shi Yan Sheng Wu Xue Bao. 2003]
[The viral resistance of transgenic tobacco plants containing untranslatable PVYN coat protein gene].
Guo XQ, Han HY, Zhang JD, Wang HG. Shi Yan Sheng Wu Xue Bao. 2003 Jun; 36(3):176-84.
Review Plant pathology and RNAi: a brief history. [Annu Rev Phytopathol. 2005]
Review Plant pathology and RNAi: a brief history.
Lindbo JA, Dougherty WG. Annu Rev Phytopathol. 2005; 43:191-204.
Review Coat protein-mediated resistance in transgenic plants. [Arch Virol. 1994]
Review Coat protein-mediated resistance in transgenic plants.
Hackland AF, Rybicki EP, Thomson JA. Arch Virol. 1994; 139(1-2):1-22.

See reviews... See all...

Cited by 20 PubMed Central articles

Antiviral RNA silencing is restricted to the marginal region of the dark green tissue in the mosaic leaves of tomato mosaic virus-infected tobacco plants. [J Virol. 2008]
Antiviral RNA silencing is restricted to the marginal region of the dark green tissue in the mosaic leaves of tomato mosaic virus-infected tobacco plants.
Hirai K, Kubota K, Mochizuki T, Tsuda S, Meshi T. J Virol. 2008 Apr; 82(7):3250-60. Epub 2008 Jan 23.
Review Plant viruses. Invaders of cells and pirates of cellular pathways. [Plant Physiol. 2005]
Review Plant viruses. Invaders of cells and pirates of cellular pathways.
Nelson RS, Citovsky V. Plant Physiol. 2005 Aug; 138(4):1809-14.
RNA interference: more than a research tool in the vertebrates' adaptive immunity. [Retrovirology. 2005]
RNA interference: more than a research tool in the vertebrates' adaptive immunity.
Mak J. Retrovirology. 2005 May 25; 2:35. Epub 2005 May 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Untranslatable transcripts of the tobacco etch virus coat protein gene sequence ...
Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts.
Virology. 1992 Aug ;189(2):725-33.

PubMed
New pathways from PKA to the Cdc2/cyclin B complex in oocytes: Wee1B as a potent...
New pathways from PKA to the Cdc2/cyclin B complex in oocytes: Wee1B as a potential PKA substrate.
Cell Cycle. 2006 Feb ;5(3):227-31. Epub 2006 Feb 8 .

PubMed
Epidemiology of human immunodeficiency virus encephalopathy in the United States...
Epidemiology of human immunodeficiency virus encephalopathy in the United States.
Neurology. 1992 Aug ;42(8):1472-6.

PubMed
A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer.
A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer.
Nucleic Acids Res. 2006 Jan 12 ;34(1):343-52. Print 2006 .

PubMed
A role for ADP-ribosylation factor in nuclear vesicle dynamics.
A role for ADP-ribosylation factor in nuclear vesicle dynamics.
Nature. 1992 Aug 6 ;358(6386):512-4.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: