J Cell Biol. 1992 Jul;118(1):63-9.

Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.

Chang WJ, Rothberg KG, Kamen BA, Anderson RG.

Source

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75235.

Abstract

The folate receptor is clustered on the surface of MA104 cells in association with caveolae. This relationship is thought to be essential for the proper internalization and recycling of the receptor during the delivery of 5-methyltetrahydrofolate to the cytoplasm of folate-depleted cells. Both the clustered organization of the receptor and the integrity of caveolae are disrupted when cells are deprived of cholesterol. We now show that cholesterol depletion of MA104 cells markedly reduces the rate of 5-methyltetrahydrofolate internalization and causes a 70% decline in the number of receptors present in the internal, recycling compartment. This effect is consistent with morphologic data showing that cholesterol-depleted MA104 cells have a reduced number of caveolae as well as fewer receptors per caveolae.

PMID:: 1618907; [PubMed - indexed for MEDLINE]
PMCID:: PMC2289528

Free PMC Article

LinkOut - more resources

Full Text Sources

Medical

Cholesterol - MedlinePlus Health Information

Molecular Biology Databases

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells. [J Cell Biol. 1996]
Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells.
Smart EJ, Mineo C, Anderson RG. J Cell Biol. 1996 Sep; 134(5):1169-77.
Fumonisin B1-induced sphingolipid depletion inhibits vitamin uptake via the glycosylphosphatidylinositol-anchored folate receptor. [J Biol Chem. 1997]
Fumonisin B1-induced sphingolipid depletion inhibits vitamin uptake via the glycosylphosphatidylinositol-anchored folate receptor.
Stevens VL, Tang J. J Biol Chem. 1997 Jul 18; 272(29):18020-5.
Review A review of folate receptor alpha cycling and 5-methyltetrahydrofolate accumulation with an emphasis on cell models in vitro. [Adv Drug Deliv Rev. 2004]
Review A review of folate receptor alpha cycling and 5-methyltetrahydrofolate accumulation with an emphasis on cell models in vitro.
Kamen BA, Smith AK. Adv Drug Deliv Rev. 2004 Apr 29; 56(8):1085-97.
Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae. [J Cell Biol. 1994]
Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae.
Smart EJ, Foster DC, Ying YS, Kamen BA, Anderson RG. J Cell Biol. 1994 Feb; 124(3):307-13.
Review [Folate receptor and its application in the selective receptor-mediated targeting therapy of tumor cells--review]. [Zhongguo Shi Yan Xue Ye Xue Za...]
Review [Folate receptor and its application in the selective receptor-mediated targeting therapy of tumor cells--review].
Gao XN, Tang SQ. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Oct; 13(5):911-4.

See reviews... See all...

Cited by 43 PubMed Central articles

Impact of oxLDL on Cholesterol-Rich Membrane Rafts. [J Lipids. 2011]
Impact of oxLDL on Cholesterol-Rich Membrane Rafts.
Levitan I, Shentu TP. J Lipids. 2011; 2011:730209. Epub 2011 Jan 18.
Enhanced laser thermal ablation for the in vitro treatment of liver cancer by specific delivery of multiwalled carbon nanotubes functionalized with human serum albumin. [Int J Nanomedicine. 2011]
Enhanced laser thermal ablation for the in vitro treatment of liver cancer by specific delivery of multiwalled carbon nanotubes functionalized with human serum albumin.
Iancu C, Mocan L, Bele C, Orza AI, Tabaran FA, Catoi C, Stiufiuc R, Stir A, Matea C, Iancu D, et al. Int J Nanomedicine. 2011 Jan 17; 6:129-41. Epub 2011 Jan 17.
Plasma folate levels are associated with the lipoprotein profile: a retrospective database analysis. [Nutr J. 2010]
Plasma folate levels are associated with the lipoprotein profile: a retrospective database analysis.
Semmler A, Moskau S, Grigull A, Farmand S, Klockgether T, Smulders Y, Blom H, Zur B, Stoffel-Wagner B, Linnebank M. Nutr J. 2010 Jul 28; 9:31. Epub 2010 Jul 28.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Lowering the cholesterol content of MA104 cells inhibits receptor-mediated trans...
Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.
J Cell Biol. 1992 Jul ;118(1):63-9.

PubMed
Reduced diversity of faecal microbiota in Crohn's disease revealed by a metageno...
Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach.
Gut. 2006 Feb ;55(2):205-11. Epub 2005 Sep 27 .

PubMed
Blood vitamin concentrations during the acute-phase response.
Blood vitamin concentrations during the acute-phase response.
Crit Care Med. 1992 Jul ;20(7):934-41.

PubMed
Obesity: basic science and medical aspects relevant to anaesthetists.
Obesity: basic science and medical aspects relevant to anaesthetists.
Anaesthesia. 2005 Oct ;60(10):1009-21.

PubMed
Inhibition of tobacco-specific nitrosamine-induced lung tumorigenesis in A/J mic...
Inhibition of tobacco-specific nitrosamine-induced lung tumorigenesis in A/J mice by green tea and its major polyphenol as antioxidants.
Cancer Res. 1992 Jul 15 ;52(14):3875-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Medical

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 43 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information