Nucleic Acids Res. 1992 Jun 11;20(11):2773-6.

The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving.

Source

Arnold and Mabel Beckman Laboratories of Chemical Synthesis, California Institute of Technology, Pasadena 91125.

Abstract

The influence of sixteen base triplet changes at a single position within a pur.pur.pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G.GC, A.AT and T.AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25 degrees C). Weaker interactions were detected for the C.AT, A.GC and T.CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.

PMID:: 1614865; [PubMed - indexed for MEDLINE]
PMCID:: PMC336921

Free PMC Article

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

HG00329/HG/NHGRI NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Specific recognition of CG base pairs by 2-deoxynebularine within the purine.purine.pyrimidine triple-helix motif. [Biochemistry. 1993]
Specific recognition of CG base pairs by 2-deoxynebularine within the purine.purine.pyrimidine triple-helix motif.
Stilz HU, Dervan PB. Biochemistry. 1993 Mar 9; 32(9):2177-85.
Elucidation of the sequence-specific third-strand recognition of four Watson-Crick base pairs in a pyrimidine triple-helix motif: T.AT, C.GC, T.CG, and G.TA. [Proc Natl Acad Sci U S A. 1992]
Elucidation of the sequence-specific third-strand recognition of four Watson-Crick base pairs in a pyrimidine triple-helix motif: T.AT, C.GC, T.CG, and G.TA.
Yoon K, Hobbs CA, Koch J, Sardaro M, Kutny R, Weis AL. Proc Natl Acad Sci U S A. 1992 May 1; 89(9):3840-4.
DNA triple helix formation at oligopurine sites containing multiple contiguous pyrimidines. [Nucleic Acids Res. 1997]
DNA triple helix formation at oligopurine sites containing multiple contiguous pyrimidines.
Gowers DM, Fox KR. Nucleic Acids Res. 1997 Oct 1; 25(19):3787-94.
Review Targeting DNA base pair mismatch with artificial nucleobases. Advances and perspectives in triple helix strategy. [Org Biomol Chem. 2011]
Review Targeting DNA base pair mismatch with artificial nucleobases. Advances and perspectives in triple helix strategy.
Malnuit V, Duca M, Benhida R. Org Biomol Chem. 2011 Jan 21; 9(2):326-36. Epub 2010 Nov 3.
Review The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences. [FASEB J. 1988]
Review The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences.
Wells RD, Collier DA, Hanvey JC, Shimizu M, Wohlrab F. FASEB J. 1988 Nov; 2(14):2939-49.

See reviews... See all...

Cited by 31 PubMed Central articles

Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing. [Nucleic Acids Res. 2011]
Reconstitution of a functional IS608 single-strand transpososome: role of non-canonical base pairing.
He S, Hickman AB, Dyda F, Johnson NP, Chandler M, Ton-Hoang B. Nucleic Acids Res. 2011 Oct; 39(19):8503-12. Epub 2011 Jul 10.
Recognition of Single-Stranded Nucleic Acids by Triplex Formation: The Binding of Pyrimidine-Rich Sequences. [J Am Chem Soc. 1994]
Recognition of Single-Stranded Nucleic Acids by Triplex Formation: The Binding of Pyrimidine-Rich Sequences.
Wang S, Kool ET. J Am Chem Soc. 1994 Sep; 116(19):8857-8858.
Selective inhibition of the human tie-1 promoter with triplex-forming oligonucleotides targeted to Ets binding sites. [Mol Med. 2006]
Selective inhibition of the human tie-1 promoter with triplex-forming oligonucleotides targeted to Ets binding sites.
Hewett PW, Daft EL, Laughton CA, Ahmad S, Ahmed A, Murray JC. Mol Med. 2006 Jan-Mar; 12(1-3):8-16.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The influence of single base triplet changes on the stability of a pur.pur.pyr t...
The influence of single base triplet changes on the stability of a pur.pur.pyr triple helix determined by affinity cleaving.
Nucleic Acids Res. 1992 Jun 11 ;20(11):2773-6.

PubMed
Effects of probiotics on the gastrointestinal tract.
Effects of probiotics on the gastrointestinal tract.
Curr Opin Infect Dis. 2005 Oct ;18(5):420-6.

PubMed
Objective analysis of the topological organization of the primate cortical visua...
Objective analysis of the topological organization of the primate cortical visual system.
Nature. 1992 Jul 9 ;358(6382):152-5.

PubMed
Multilocus sequence typing scheme that provides both species and strain differen...
Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.
J Clin Microbiol. 2005 Sep ;43(9):4665-73.

PubMed
Clinical trials and meta-analysis. What do they do for us?
Clinical trials and meta-analysis. What do they do for us?
N Engl J Med. 1992 Jul 23 ;327(4):273-4.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: