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    Science. 2005 Aug 19;309(5738):1248-51. Epub 2005 Jul 14.

    Genome-wide RNAi screen for host factors required for intracellular bacterial infection.

    Agaisse H, Burrack LS, Philips JA, Rubin EJ, Perrimon N, Higgins DE.

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    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. herve.agaisse@yale.edu

    Abstract

    Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.

    PMID:
    16020693
    [PubMed - indexed for MEDLINE]

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