Connect Tissue Res. 2005;46(2):83-91.

The concentration, gene expression, and spatial distribution of aggrecan in canine articular cartilage, meniscus, and anterior and posterior cruciate ligaments: a new molecular distinction between hyaline cartilage and fibrocartilage in the knee joint.

Source

Department of Biomedical Engineering, Lerner Research Institute and Orthopaedic Research Center, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.

Abstract

The concentration, spatial distribution, and gene expression of aggrecan in meniscus, articular cartilage, and the anterior and posterior cruciate ligaments (ACL and PCL) was determined in the knee joints of five mature dogs. An anti-serum against peptide sequences specific to the G1 domain of aggrecan was employed in competitive-inhibition ELISA of guanidine HCl extracts and immunofluorescence microscopy. Gene expression was determined by Taqman real-time PCR. The concentration of aggrecan in articular cartilage (240.1 +/- 32 nMol/g dry weight) was higher than that in meniscus (medial meniscus: 33.4 +/- 4.3 nMol/g) and ligaments (ACL: 6.8 +/- 0.9 nMol/g). Aggrecan was more concentrated in the inner than the outer zone of the meniscus. Aggrecan in meniscus showed an organized, spatial network, in contrast to its diffuse distribution in articular cartilage. Thus, differences in the concentration, gene expression, and spatial distribution of aggrecan constitute another molecular distinction between hyaline cartilage and fibrocartilage of the knee.

PMID:: 16019418; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage. [Osteoarthritis Cartilage. 2001]
Age-related changes in the synthesis and mRNA expression of decorin and aggrecan in human meniscus and articular cartilage.
McAlinden A, Dudhia J, Bolton MC, Lorenzo P, Heinegård D, Bayliss MT. Osteoarthritis Cartilage. 2001 Jan; 9(1):33-41.
Knee joint immobilization decreases aggrecan gene expression in the meniscus. [Am J Sports Med. 1998]
Knee joint immobilization decreases aggrecan gene expression in the meniscus.
Djurasovic M, Aldridge JW, Grumbles R, Rosenwasser MP, Howell D, Ratcliffe A. Am J Sports Med. 1998 May-Jun; 26(3):460-6.
Changes in joint cartilage aggrecan after knee injury and in osteoarthritis. [Arthritis Rheum. 1999]
Changes in joint cartilage aggrecan after knee injury and in osteoarthritis.
Lohmander LS, Ionescu M, Jugessur H, Poole AR. Arthritis Rheum. 1999 Mar; 42(3):534-44.
Review Aggrecan, aging and assembly in articular cartilage. [Cell Mol Life Sci. 2005]
Review Aggrecan, aging and assembly in articular cartilage.
Dudhia J. Cell Mol Life Sci. 2005 Oct; 62(19-20):2241-56.
Review Anatomy of the posterior cruciate ligament and the meniscofemoral ligaments. [Knee Surg Sports Traumatol Art...]
Review Anatomy of the posterior cruciate ligament and the meniscofemoral ligaments.
Amis AA, Gupte CM, Bull AM, Edwards A. Knee Surg Sports Traumatol Arthrosc. 2006 Mar; 14(3):257-63. Epub 2005 Oct 14.

See reviews... See all...

Cited by 11 PubMed Central articles

A novel and non-destructive method to examine meniscus architecture using 9.4 Tesla MRI. [Osteoarthritis Cartilage. 2010]
A novel and non-destructive method to examine meniscus architecture using 9.4 Tesla MRI.
Wang M, Radjenovic A, Stapleton TW, Venkatesh R, Williams S, Ingham E, Fisher J, Jin Z. Osteoarthritis Cartilage. 2010 Nov; 18(11):1417-20. Epub 2010 Aug 24.
Meniscus and cartilage exhibit distinct intra-tissue strain distributions under unconfined compression. [Osteoarthritis Cartilage. 2010]
Meniscus and cartilage exhibit distinct intra-tissue strain distributions under unconfined compression.
Lai JH, Levenston ME. Osteoarthritis Cartilage. 2010 Oct; 18(10):1291-9. Epub 2010 Jul 13.
Tissue engineering by molecular disassembly and reassembly: biomimetic retention of mechanically functional aggrecan in hydrogel. [Tissue Eng Part C Methods. 2010]
Tissue engineering by molecular disassembly and reassembly: biomimetic retention of mechanically functional aggrecan in hydrogel.
Han E, Wilensky LM, Schumacher BL, Chen AC, Masuda K, Sah RL. Tissue Eng Part C Methods. 2010 Dec; 16(6):1471-9. Epub 2010 Jun 9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The concentration, gene expression, and spatial distribution of aggrecan in cani...
The concentration, gene expression, and spatial distribution of aggrecan in canine articular cartilage, meniscus, and anterior and posterior cruciate ligaments: a new molecular distinction between hyaline cartilage and fibrocartilage in the knee joint.
Connect Tissue Res. 2005 ;46(2):83-91.

PubMed
Thrombospondin 3 (Thbs3), a new member of the thrombospondin gene family.
Thrombospondin 3 (Thbs3), a new member of the thrombospondin gene family.
J Biol Chem. 1992 Jun 15 ;267(17):12192-6.

PubMed
Genetic variations in the human gelatinase A (matrix metalloproteinase-2) promot...
Genetic variations in the human gelatinase A (matrix metalloproteinase-2) promoter are not associated with susceptibility to, and severity of, chronic periodontitis.
J Periodontol. 2005 Jul ;76(7):1056-60.

PubMed
Leukocyte chemotactic activity of cyclophilin.
Leukocyte chemotactic activity of cyclophilin.
J Biol Chem. 1992 Jun 15 ;267(17):11968-71.

PubMed
Successful treatment of acute visual loss in Muckle-Wells syndrome with interleu...
Successful treatment of acute visual loss in Muckle-Wells syndrome with interleukin 1 receptor antagonist.
Ann Rheum Dis. 2005 Aug ;64(8):1245-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: