J Bacteriol. 1992 May;174(9):2779-84.

The lrp gene product regulates expression of lysU in Escherichia coli K-12.

Lin R, Ernsting B, Hirshfield IN, Matthews RG, Neidhardt FC, Clark RL, Newman EB.

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Biology Department, Concordia University, Montreal, Quebec, Canada.

Abstract

In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that LysU is present at higher levels in an lrp strain than in its isogenic lrp+ parent. The purified lrp gene product was shown to bind to sites upstream of the lysU gene and to protect several sites against DNase I digestion. A region extending over 100 nucleotides, between 60 and 160 nucleotides upstream from the start of the lysU coding sequence, showed altered sensitivity to DNase I digestion in the presence of the Lrp protein. The extent of protected DNA suggests a complex interaction of Lrp protein and upstream lysU DNA.

PMID:: 1569010; [PubMed - indexed for MEDLINE]
PMCID:: PMC205928

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The lrp gene product regulates expression of lysU in Escherichia coli K-12.

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