Science. 1992 Apr 17;256(5055):379-82.

Reciprocal regulation of adipogenesis by Myc and C/EBP alpha.

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Molecular Biology Research Program, Henry Ford Hospital, Detroit, MI 48202.

Abstract

3T3-L1 adipoblasts that express large amounts of c-Myc cannot terminally differentiate, raising the possibility that Myc inhibits the expression of genes that promote adipogenesis. The CCAAT/enhancer binding protein (C/EBP alpha) is induced during 3T3-L1 adipogenesis when cells commit to the differentiation pathway. Transfection of 3T3-L1 adipoblasts with the gene that encodes C/EBP alpha caused overt expression of the adipocyte morphology. Expression of Myc prohibited the normal induction of C/EBP alpha and prevented adipogenesis. Enforced expression of C/EBP alpha overcame the Myc-induced block to differentiation. These results provide a molecular basis for the regulation of adipogenesis and implicate Myc and C/EBP alpha as pivotal controlling elements.

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Reciprocal regulation of adipogenesis by Myc and C/EBP alpha.

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