Lancet Infect Dis. 2004 Dec;4(12):751-60.

New developments in the diagnosis of bloodstream infections.

Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM.

Source

Department of Internal Medicine, VU University Medical Center, Amsterdam, Netherlands.

Abstract

New techniques have emerged for the detection of bacteria in blood, because the blood culture as gold standard is slow and insufficiently sensitive when the patient has previously received antibiotics or in the presence of fastidious organisms. DNA-based techniques, hybridisation probes, and PCR-based detection or protein-based detection by mass spectroscopy are aimed at rapid identification of bacteria and provide results within 2 h after the first signal of growth in conventional blood cultures. Also, detection of microorganisms directly in blood by pathogen-specific or broad-range PCR assays (eubacterial or panfungal) shows promising results. Interpretation is complex, however, because of detection of DNA rather than living pathogens, the risk of interfering contamination, the presence of background DNA in blood, and the lack of a gold standard. As these techniques are emerging, clinical value and cost-effectiveness have to be assessed. Nevertheless, molecular assays are expected eventually to replace the current conventional microbiological techniques for detection of bloodstream infections.

PMID:: 15567125; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Review Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria. [Int J Antimicrob Agents. 2007]
Review Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria.
Fenollar F, Raoult D. Int J Antimicrob Agents. 2007 Nov; 30 Suppl 1:S7-15. Epub 2007 Aug 17.
Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene. [J Clin Microbiol. 2000]
Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene.
Turenne CY, Witwicki E, Hoban DJ, Karlowsky JA, Kabani AM. J Clin Microbiol. 2000 Feb; 38(2):513-20.
Review Rapid methods for diagnosis of bloodstream infections. [Clin Chem Lab Med. 2008]
Review Rapid methods for diagnosis of bloodstream infections.
Klouche M, Schröder U. Clin Chem Lab Med. 2008; 46(7):888-908.
A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. [Med Microbiol Immunol. 2008]
A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples.
Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A, Stüber F. Med Microbiol Immunol. 2008 Sep; 197(3):313-24. Epub 2007 Nov 16.
Characterisation of prototype Nurmi cultures using culture-based microbiological techniques and PCR-DGGE. [Int J Food Microbiol. 2006]
Characterisation of prototype Nurmi cultures using culture-based microbiological techniques and PCR-DGGE.
Waters SM, Murphy RA, Power RF. Int J Food Microbiol. 2006 Aug 1; 110(3):268-77. Epub 2006 Jul 11.

See reviews... See all...

Cited by 35 PubMed Central articles

Ten-year surveillance of nosocomial bloodstream infections: trends of aetiology and antimicrobial resistance in a comprehensive cancer centre. [Ecancermedicalscience. 2011]
Ten-year surveillance of nosocomial bloodstream infections: trends of aetiology and antimicrobial resistance in a comprehensive cancer centre.
Passerini R, Ghezzi T, Sandri M, Radice D, Biffi R. Ecancermedicalscience. 2011; 5:191. Epub 2011 Feb 17.
Accuracy of LightCycler(R) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol. [BMJ Open. 2012]
Accuracy of LightCycler(R) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol.
Dark P, Wilson C, Blackwood B, McAuley DF, Perkins GD, McMullan R, Gates S, Warhurst G. BMJ Open. 2012; 2(1):e000392. Epub 2012 Jan 12.
Proteomics mapping of cord blood identifies haptoglobin "switch-on" pattern as biomarker of early-onset neonatal sepsis in preterm newborns. [PLoS One. 2011]
Proteomics mapping of cord blood identifies haptoglobin "switch-on" pattern as biomarker of early-onset neonatal sepsis in preterm newborns.
Buhimschi CS, Bhandari V, Dulay AT, Nayeri UA, Abdel-Razeq SS, Pettker CM, Thung S, Zhao G, Han YW, Bizzarro M, et al. PLoS One. 2011; 6(10):e26111. Epub 2011 Oct 10.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

New developments in the diagnosis of bloodstream infections.
New developments in the diagnosis of bloodstream infections.
Lancet Infect Dis. 2004 Dec ;4(12):751-60.

PubMed
The adult respiratory distress syndrome. A report of survival and modifying fact...
The adult respiratory distress syndrome. A report of survival and modifying factors.
Chest. 1992 Apr ;101(4):1074-9.

PubMed
Intensive care unit utilization and interhospital transfers as potential indicat...
Intensive care unit utilization and interhospital transfers as potential indicators of rural hospital quality.
J Rural Health. 2004 Fall ;20(4):394-400.

PubMed
Comparison of etomidate, ketamine, midazolam, propofol, and thiopental on functi...
Comparison of etomidate, ketamine, midazolam, propofol, and thiopental on function and metabolism of isolated hearts.
Anesth Analg. 1992 Apr ;74(4):547-58.

PubMed
Atorvastatin reduction of intravascular thrombosis, increase in cerebral microva...
Atorvastatin reduction of intravascular thrombosis, increase in cerebral microvascular patency and integrity, and enhancement of spatial learning in rats subjected to traumatic brain injury.
J Neurosurg. 2004 Nov ;101(5):813-21.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

New developments in the diagnosis of bloodstream infections.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 35 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information