Science. 2004 Nov 12;306(5699):1194-8.

Multidimensional drug profiling by automated microscopy.

Perlman ZE, Slack MD, Feng Y, Mitchison TJ, Wu LF, Altschuler SJ.

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Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiling at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.

PMID:: 15539606; [PubMed - indexed for MEDLINE]

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Multidimensional drug profiling by automated microscopy.

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