Science. 1992 Jan 10;255(5041):195-7.

Biological significance of unwinding capability of nuclear matrix-associating DNAs.

Bode J, Kohwi Y, Dickinson L, Joh T, Klehr D, Mielke C, Kohwi-Shigematsu T.

Source

Gesellschaft für Biotechnologische Forschung, mbH, Genetik von Eukaryoten, Mascheroder Weg 1, Braunschweig-Stöckheim, Federal Republic of Germany.

Abstract

Matrix attachment regions (MARs) are thought to separate chromatin into topologically constrained loop domains. A MAR located 5' of the human beta-interferon gene becomes stably base-unpaired under superhelical strain, as do the MARs flanking the immunoglobulin heavy chain gene enhancer; in both cases a nucleation site exists for DNA unwinding. Concatemerized oligonucleotides containing the unwinding nucleation site exhibited a strong affinity for the nuclear scaffold and augmented SV40 promoter activity in stable transformants. Mutated concatemerized oligonucleotides resisted unwinding, showed weak affinity for the nuclear scaffold, and did not enhance promoter activity. These results suggest that the DNA feature capable of relieving superhelical strain is important for MAR functions.

PMID:: 1553545; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Torsional stress stabilizes extended base unpairing in suppressor sites flanking immunoglobulin heavy chain enhancer. [Biochemistry. 1990]
Torsional stress stabilizes extended base unpairing in suppressor sites flanking immunoglobulin heavy chain enhancer.
Kohwi-Shigematsu T, Kohwi Y. Biochemistry. 1990 Oct 16; 29(41):9551-60.
Nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses. [J Virol. 1998]
Nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses.
Tan SH, Bartsch D, Schwarz E, Bernard HU. J Virol. 1998 May; 72(5):3610-22.
Nuclear matrix attachment regions of human papillomavirus type 16 repress or activate the E6 promoter, depending on the physical state of the viral DNA. [J Virol. 2000]
Nuclear matrix attachment regions of human papillomavirus type 16 repress or activate the E6 promoter, depending on the physical state of the viral DNA.
Stünkel W, Huang Z, Tan SH, O'Connor MJ, Bernard HU. J Virol. 2000 Mar; 74(6):2489-501.
Review Chromatin domains and prediction of MAR sequences. [Int Rev Cytol. 1995]
Review Chromatin domains and prediction of MAR sequences.
Boulikas T. Int Rev Cytol. 1995; 162A:279-388.
Review Scaffold/matrix-attached regions: structural properties creating transcriptionally active loci. [Int Rev Cytol. 1995]
Review Scaffold/matrix-attached regions: structural properties creating transcriptionally active loci.
Bode J, Schlake T, Ríos-Ramírez M, Mielke C, Stengert M, Kay V, Klehr-Wirth D. Int Rev Cytol. 1995; 162A:389-454.

See reviews... See all...

Cited by 70 PubMed Central articles

The structural basis for the oligomerization of the N-terminal domain of SATB1. [Nucleic Acids Res. 2012]
The structural basis for the oligomerization of the N-terminal domain of SATB1.
Wang Z, Yang X, Chu X, Zhang J, Zhou H, Shen Y, Long J. Nucleic Acids Res. 2012 May 1; 40(9):4193-202. Epub 2012 Jan 12.
Satb1 ablation alters temporal expression of immediate early genes and reduces dendritic spine density during postnatal brain development. [Mol Cell Biol. 2012]
Satb1 ablation alters temporal expression of immediate early genes and reduces dendritic spine density during postnatal brain development.
Balamotis MA, Tamberg N, Woo YJ, Li J, Davy B, Kohwi-Shigematsu T, Kohwi Y. Mol Cell Biol. 2012 Jan; 32(2):333-47. Epub 2011 Nov 7.
p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis. [J Cell Biol. 2011]
p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis.
Fessing MY, Mardaryev AN, Gdula MR, Sharov AA, Sharova TY, Rapisarda V, Gordon KB, Smorodchenko AD, Poterlowicz K, Ferone G, et al. J Cell Biol. 2011 Sep 19; 194(6):825-39.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Biological significance of unwinding capability of nuclear matrix-associating DN...
Biological significance of unwinding capability of nuclear matrix-associating DNAs.
Science. 1992 Jan 10 ;255(5041):195-7.

PubMed
Enabling the exploration of biochemical pathways.
Enabling the exploration of biochemical pathways.
Org Biomol Chem. 2004 Nov 21 ;2(22):3226-37. Epub 2004 Oct 22 .

PubMed
A statistical test for detecting geographic subdivision.
A statistical test for detecting geographic subdivision.
Mol Biol Evol. 1992 Jan ;9(1):138-51.

PubMed
A general survey of thymocyte differentiation by transcriptional analysis of kno...
A general survey of thymocyte differentiation by transcriptional analysis of knockout mouse models.
J Immunol. 2004 Nov 15 ;173(10):6109-18.

PubMed
Reassociation kinetics of Anopheles gambiae (Diptera: Culicidae) DNA.
Reassociation kinetics of Anopheles gambiae (Diptera: Culicidae) DNA.
J Med Entomol. 1992 Jan ;29(1):125-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Biological significance of unwinding capability of nuclear matrix-associating DNAs.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 70 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information