Nature. 1992 Mar 26;356(6367):336-8.

Rhodopsin inactivation is a modulated process in Limulus photoreceptors.

Source

Department of Biology, Brandeis University, Waltham, Massachusetts 02254.

Abstract

Many G-protein-coupled receptors are only transiently active because an inactivation process stops the receptor from activating G protein molecules. Although this inactivation has been investigated in vitro, the real kinetics of the process can only be obtained from intact cells. Here we describe a method for measuring the inactivation of rhodopsin in intact photoreceptors and the application of this method to the ultraviolet rhodopsin of Limulus median eye. The results show that the inactivation process is very rapid (less than 150 ms) and occurs well before the peak of the receptor potential. We have also investigated whether the inactivation process can itself be modulated. Our results show that light-adaptation accelerates inactivation by about 10-fold, providing evidence that G-protein-mediated transduction can be modulated at this first stage.

PMID:: 1549176; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Rhodopsin

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Nontransducing rhodopsin. [J Gen Physiol. 1987]
Nontransducing rhodopsin.
Levine E, Crain E, Robinson P, Lisman J. J Gen Physiol. 1987 Oct; 90(4):575-86.
An estimate of the number of G regulator proteins activated per excited rhodopsin in living Limulus ventral photoreceptors. [Proc Natl Acad Sci U S A. 1989]
An estimate of the number of G regulator proteins activated per excited rhodopsin in living Limulus ventral photoreceptors.
Kirkwood A, Weiner D, Lisman JE. Proc Natl Acad Sci U S A. 1989 May; 86(10):3872-6.
Early events in visual transduction in Limulus photoreceptors. [Neurosci Res Suppl. 1985]
Early events in visual transduction in Limulus photoreceptors.
Lisman J, Goldring M. Neurosci Res Suppl. 1985; 2:S101-17.
Review Biophysical processes in invertebrate photoreceptors: recent progress and a critical overview based on Limulus photoreceptors. [Q Rev Biophys. 1991]
Review Biophysical processes in invertebrate photoreceptors: recent progress and a critical overview based on Limulus photoreceptors.
Nagy K. Q Rev Biophys. 1991 May; 24(2):165-226.
Review Mechanisms of amplification, deactivation, and noise reduction in invertebrate photoreceptors. [Soc Gen Physiol Ser. 1992]
Review Mechanisms of amplification, deactivation, and noise reduction in invertebrate photoreceptors.
Lisman J, Erickson MA, Richard EA, Cote RH, Bacigalupo J, Johnson E, Kirkwood A. Soc Gen Physiol Ser. 1992; 47:175-99.

See reviews... See all...

Cited by 8 PubMed Central articles

Role of Phosphorylation in the Control of Clathrin-Mediated Internalization of GPCR. [Int J Cell Biol. 2011]
Role of Phosphorylation in the Control of Clathrin-Mediated Internalization of GPCR.
Delom F, Fessart D. Int J Cell Biol. 2011; 2011:246954. Epub 2011 Jun 7.
Ca2+-dependent metarhodopsin inactivation mediated by calmodulin and NINAC myosin III. [Neuron. 2008]
Ca2+-dependent metarhodopsin inactivation mediated by calmodulin and NINAC myosin III.
Liu CH, Satoh AK, Postma M, Huang J, Ready DF, Hardie RC. Neuron. 2008 Sep 11; 59(5):778-89.
Translocation of Gq alpha mediates long-term adaptation in Drosophila photoreceptors. [J Neurosci. 2007]
Translocation of Gq alpha mediates long-term adaptation in Drosophila photoreceptors.
Frechter S, Elia N, Tzarfaty V, Selinger Z, Minke B. J Neurosci. 2007 May 23; 27(21):5571-83.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Rhodopsin inactivation is a modulated process in Limulus photoreceptors.
Rhodopsin inactivation is a modulated process in Limulus photoreceptors.
Nature. 1992 Mar 26 ;356(6367):336-8.

PubMed
In vivo dynamics of an immune response in the bumble bee Bombus terrestris.
In vivo dynamics of an immune response in the bumble bee Bombus terrestris.
J Invertebr Pathol. 2004 Sep ;87(1):59-66.

PubMed
Acquired syphilis in adults.
Acquired syphilis in adults.
N Engl J Med. 1992 Apr 16 ;326(16):1060-9.

PubMed
Effect of splenectomy on type-1/type-2 cytokine gene expression in a patient wit...
Effect of splenectomy on type-1/type-2 cytokine gene expression in a patient with adult idiopathic thrombocytopenic purpura (ITP).
BMC Blood Disord. 2004 Oct 18;4(1):4.

PubMed
Glucose repression of the yeast ADH2 gene occurs through multiple mechanisms, in...
Glucose repression of the yeast ADH2 gene occurs through multiple mechanisms, including control of the protein synthesis of its transcriptional activator, ADR1.
Mol Cell Biol. 1992 Apr ;12(4):1663-73.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: