Cell Calcium. 2004 Dec;36(6):509-14.

Novel fluo-4 analogs for fluorescent calcium measurements.

Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.

Source

Molecular Probes Invitrogen Detection Technologies, 29851 Willow Creek Rd, Eugene, OR 97492, USA.

Abstract

We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.

PMID:: 15488600; [PubMed - indexed for MEDLINE]

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