Science. 1992 Mar 6;255(5049):1261-3.

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.

Hunt DF, Henderson RA, Shabanowitz J, Sakaguchi K, Michel H, Sevilir N, Cox AL, Appella E, Engelhard VH.

Source

Department of Chemistry, University of Virginia, Charlottesville 22903.

Abstract

Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.

Comment on

Science. 1992 Mar 6;255(5049):1214-5.

PMID:: 1546328; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

HLA-A2.1-associated peptides from a mutant cell line: a second pathway of antigen presentation. [Science. 1992]
HLA-A2.1-associated peptides from a mutant cell line: a second pathway of antigen presentation.
Henderson RA, Michel H, Sakaguchi K, Shabanowitz J, Appella E, Hunt DF, Engelhard VH. Science. 1992 Mar 6; 255(5049):1264-6.
Pillars article: Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 1992. 255: 1261-1263. [J Immunol. 2007]
Pillars article: Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 1992. 255: 1261-1263.
Hunt DF, Henderson RA, Shabanowitz J, Sakaguchi K, Michel H, Sevilir N, Cox AL, Appella E, Engelhard VH. J Immunol. 2007 Sep 1; 179(5):2669-71.
Direct identification of an endogenous peptide recognized by multiple HLA-A2.1-specific cytotoxic T cells. [Proc Natl Acad Sci U S A. 1993]
Direct identification of an endogenous peptide recognized by multiple HLA-A2.1-specific cytotoxic T cells.
Henderson RA, Cox AL, Sakaguchi K, Appella E, Shabanowitz J, Hunt DF, Engelhard VH. Proc Natl Acad Sci U S A. 1993 Nov 1; 90(21):10275-9.
Review Analysis of the structure of naturally processed peptides bound by class I and class II major histocompatibility complex molecules. [EXS. 1995]
Review Analysis of the structure of naturally processed peptides bound by class I and class II major histocompatibility complex molecules.
Appella E, Padlan EA, Hunt DF. EXS. 1995; 73:105-19.
Review Mass spectrometric analysis of peptides associated with the human class I MHC molecules HLA-A2.1 and HLA-B7 and identification of structural features that determine binding. [Chem Immunol. 1993]
Review Mass spectrometric analysis of peptides associated with the human class I MHC molecules HLA-A2.1 and HLA-B7 and identification of structural features that determine binding.
Engelhard VH, Appella E, Benjamin DC, Bodnar WM, Cox AL, Chen Y, Henderson RA, Huczko EL, Michel H, Sakaguichi K. Chem Immunol. 1993; 57:39-62.

See reviews... See all...

Cited by over 100 PubMed Central articles

POPISK: T-cell reactivity prediction using support vector machines and string kernels. [BMC Bioinformatics. 2011]
POPISK: T-cell reactivity prediction using support vector machines and string kernels.
Tung CW, Ziehm M, Kämper A, Kohlbacher O, Ho SY. BMC Bioinformatics. 2011 Nov 15; 12:446. Epub 2011 Nov 15.
Homology-Driven Proteomics of Dinoflagellates with Unsequenced Genomes Using MALDI-TOF/TOF and Automated De Novo Sequencing. [Evid Based Complement Alternat...]
Homology-Driven Proteomics of Dinoflagellates with Unsequenced Genomes Using MALDI-TOF/TOF and Automated De Novo Sequencing.
Wang DZ, Li C, Xie ZX, Dong HP, Lin L, Hong HS. Evid Based Complement Alternat Med. 2011; 2011:471020. Epub 2011 Sep 29.
The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation. [Mol Syst Biol. 2011]
The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.
Caron E, Vincent K, Fortier MH, Laverdure JP, Bramoullé A, Hardy MP, Voisin G, Roux PP, Lemieux S, Thibault P, et al. Mol Syst Biol. 2011 Sep 27; 7:533. Epub 2011 Sep 27.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass ...
Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.
Science. 1992 Mar 6 ;255(5049):1261-3.

PubMed
Glutathione S-transferases - potential components of anti-schistosome vaccines?
Glutathione S-transferases - potential components of anti-schistosome vaccines?
Parasitol Today. 1989 Feb ;5(2):34-7.

PubMed
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in ...
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
Science. 1992 Feb 21 ;255(5047):991-4.

PubMed
Sex-specific selection on the human X chromosome?
Sex-specific selection on the human X chromosome?
Genet Res. 2004 Jun ;83(3):169-76.

PubMed
Origin of the mutual activation of the alpha and beta 2 subunits in the alpha 2 ...
Origin of the mutual activation of the alpha and beta 2 subunits in the alpha 2 beta 2 complex of tryptophan synthase. Effect of alanine or glycine substitutions at proline residues in the alpha subunit.
J Biol Chem. 1992 Mar 15 ;267(8):5222-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.

Source

Abstract

Comment on

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by over 100 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information