EMBO J. 1992 Feb;11(2):733-40.

DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae.

Araki H, Ropp PA, Johnson AL, Johnston LH, Morrison A, Sugino A.

Source

Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina.

Abstract

Two temperature-sensitive DNA polymerase II mutants (pol2-9 and pol2-18) of the yeast Saccharomyces cerevisiae were isolated by the plasmid shuffling method. DNA polymerase II activity partially purified from both mutants was thermolabile, while DNA polymerase I and III activities remained thermotolerant. At the restrictive temperature, the pol2 mutants were defective in chromosomal DNA replication and exhibited the dumbbell terminal morphology typical of DNA replication mutants. The POL2 transcript accumulated periodically during the cell cycle, peaking at the G1/S boundary in the same manner as the transcripts of more than 10 other DNA replication genes. These results indicate that DNA polymerase II participates in nuclear DNA replication. The similarities in structure and activities between the DNA polymerases of yeast and mammals make it likely that mammalian DNA polymerase epsilon too is required for chromosomal DNA replication.

PMID:: 1537345; [PubMed - indexed for MEDLINE]
PMCID:: PMC556506

Free PMC Article

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Saccharomyces Genome Database

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe. [Genes Cells. 1998]
DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe.
Sugino A, Ohara T, Sebastian J, Nakashima N, Araki H. Genes Cells. 1998 Feb; 3(2):99-110.
Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion. [Mol Cell Biol. 2003]
Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion.
Edwards S, Li CM, Levy DL, Brown J, Snow PM, Campbell JL. Mol Cell Biol. 2003 Apr; 23(8):2733-48.
DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae. [Proc Natl Acad Sci U S A. 1991]
DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae.
Araki H, Hamatake RK, Johnston LH, Sugino A. Proc Natl Acad Sci U S A. 1991 Jun 1; 88(11):4601-5.
Review Yeast DNA polymerases and their role at the replication fork. [Trends Biochem Sci. 1995]
Review Yeast DNA polymerases and their role at the replication fork.
Sugino A. Trends Biochem Sci. 1995 Aug; 20(8):319-23.
Review DNA polymerase II, the epsilon polymerase of Saccharomyces cerevisiae. [Prog Nucleic Acid Res Mol Biol...]
Review DNA polymerase II, the epsilon polymerase of Saccharomyces cerevisiae.
Morrison A, Sugino A. Prog Nucleic Acid Res Mol Biol. 1993; 46:93-120.

See reviews... See all...

Cited by 49 PubMed Central articles

Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. [Mol Cell. 2008]
Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint.
Lou H, Komata M, Katou Y, Guan Z, Reis CC, Budd M, Shirahige K, Campbell JL. Mol Cell. 2008 Oct 10; 32(1):106-17.
Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae. [Genetics. 2008]
Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.
Jaszczur M, Flis K, Rudzka J, Kraszewska J, Budd ME, Polaczek P, Campbell JL, Jonczyk P, Fijalkowska IJ. Genetics. 2008 Feb; 178(2):633-47. Epub 2008 Feb 1.
Chromatin structure regulates gene conversion. [PLoS Biol. 2007]
Chromatin structure regulates gene conversion.
Cummings WJ, Yabuki M, Ordinario EC, Bednarski DW, Quay S, Maizels N. PLoS Biol. 2007 Oct; 5(10):e246.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, rep...
DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae.
EMBO J. 1992 Feb ;11(2):733-40.

PubMed
Idiopathic adhesive capsulitis: long-term results of conservative treatment.
Idiopathic adhesive capsulitis: long-term results of conservative treatment.
Isr Med Assoc J. 2004 Sep ;6(9):524-6.

PubMed
The mouse peroxisome proliferator activated receptor recognizes a response eleme...
The mouse peroxisome proliferator activated receptor recognizes a response element in the 5' flanking sequence of the rat acyl CoA oxidase gene.
EMBO J. 1992 Feb ;11(2):433-9.

PubMed
Comparison of prospective and retrospective methods for haplotype inference in c...
Comparison of prospective and retrospective methods for haplotype inference in case-control studies.
Genet Epidemiol. 2004 Nov ;27(3):192-201.

PubMed
Population density and cancer incidence differentials in New York State, 1978-82...
Population density and cancer incidence differentials in New York State, 1978-82.
Cancer Causes Control. 1992 Jan ;3(1):7-15.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 49 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information