Genome Biol. 2004;5(9):R71. Epub 2004 Aug 17.

A catalog of human cDNA expression clones and its application to structural genomics.

Büssow K, Quedenau C, Sievert V, Tischer J, Scheich C, Seitz H, Hieke B, Niesen FH, Götz F, Harttig U, Lehrach H.

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Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany. buessow@molgen.mpg.de

Abstract

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

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PMCID: PMC522878

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