Genes Dev. 1992 Feb;6(2):177-85.

The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.

Hiebert SW, Chellappan SP, Horowitz JM, Nevins JR.

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Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.

Abstract

Recent experiments have shown that the E2F transcription factor is in a complex with the RB1 gene product. The E2F-pRB complex can be reconstituted in an in vitro assay using a GST-RB fusion protein isolated from Escherichia coli. This interaction is dependent on pRB sequences involved in E1A/T-antigen binding as well as carboxy-terminal pRB sequences that are not necessary for E1A/T binding. Moreover, reconstitution assays reveal a requirement for an accessory factor, in addition to E2F and pRB, for formation of the E2F-pRB complex. Assays of transcription from the adenovirus E2 promoter in transfection experiments demonstrate that formation of the complex containing pRB and E2F coincides with an inhibition of E2F-dependent transcriptional activity. A mutant pRB protein that does not associate with E2F does not inhibit transcription. We conclude that as a consequence of its interaction with E2F, pRB may regulate the transcriptional function of the E2F factor.

PMID:: 1531329; [PubMed - indexed for MEDLINE]

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The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.

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