Int J Legal Med. 1992;105(2):83-6.

Identification of human remains by amplification and automated sequencing of mitochondrial DNA.

Source

Central Research and Support Establishment, Forensic Science Service, Reading, Berkshire, UK.

Abstract

The highly decomposed remains of a corpse were identified by the amplification and direct sequencing of mitochondrial (mt) DNA. Degraded DNA was extracted from bone fragments and a necrotic skin sample and amplified at 2 hypervariable segments within the mitochondrial non-coding region using 2 rounds of nested PCR. Both strands of the amplified regions were sequenced and compared with each other to ensure fidelity of the data. Sequences from the bone and skin were found to be identical and matched data generated from a blood sample provided by a presumptive sister of the deceased. Thus, this evidence was consistent with the sister and the deceased being related.

PMID:: 1520642; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Case Reports

MeSH Terms

Substances

DNA, Mitochondrial

LinkOut - more resources

Full Text Sources

EBSCO

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Identifying individuals by sequencing mitochondrial DNA from teeth. [Nat Genet. 1992]
Identifying individuals by sequencing mitochondrial DNA from teeth.
Ginther C, Issel-Tarver L, King MC. Nat Genet. 1992 Oct; 2(2):135-8.
Strategies for automated sequencing of human mitochondrial DNA directly from PCR products. [Biotechniques. 1992]
Strategies for automated sequencing of human mitochondrial DNA directly from PCR products.
Hopgood R, Sullivan KM, Gill P. Biotechniques. 1992 Jul; 13(1):82-92.
[Individual identification from extremely contaminated forensic specimens by sequencing cloned mitochondrial D-loop genes]. [Nihon Hoigaku Zasshi. 1991]
[Individual identification from extremely contaminated forensic specimens by sequencing cloned mitochondrial D-loop genes].
Tsukamoto T, Yoshii T, Yamada Y, Fukui K, Ishiyama I. Nihon Hoigaku Zasshi. 1991 Jun; 45(3):233-41.
Review DNA fingerprinting and forensic medicine. [Southeast Asian J Trop Med Pub...]
Review DNA fingerprinting and forensic medicine.
Boonsaeng V. Southeast Asian J Trop Med Public Health. 1995; 26 Suppl 1:296-300.
Review Personal identification of human remains: an overview. [J Forensic Sci. 1978]
Review Personal identification of human remains: an overview.
Warren CP. J Forensic Sci. 1978 Apr; 23(2):388-95.

See reviews... See all...

Cited by 4 PubMed Central articles

Second generation sequencing allows for mtDNA mixture deconvolution and high resolution detection of heteroplasmy. [Croat Med J. 2011]
Second generation sequencing allows for mtDNA mixture deconvolution and high resolution detection of heteroplasmy.
Holland MM, McQuillan MR, O'Hanlon KA. Croat Med J. 2011 Jun; 52(3):299-313.
Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP. [BMC Genet. 2004]
Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and deer by PCR-RFLP.
Pfeiffer I, Burger J, Brenig B. BMC Genet. 2004 Oct 5; 5:30. Epub 2004 Oct 5.
The frequency of heteroplasmy in the HVII region of mtDNA differs across tissue types and increases with age. [Am J Hum Genet. 2000]
The frequency of heteroplasmy in the HVII region of mtDNA differs across tissue types and increases with age.
Calloway CD, Reynolds RL, Herrin GL Jr, Anderson WW. Am J Hum Genet. 2000 Apr; 66(4):1384-97. Epub 2000 Mar 17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Identification of human remains by amplification and automated sequencing of mit...
Identification of human remains by amplification and automated sequencing of mitochondrial DNA.
Int J Legal Med. 1992 ;105(2):83-6.

PubMed
Polyclonal immunoglobulin for treatment of bacterial sepsis: a systematic review...
Polyclonal immunoglobulin for treatment of bacterial sepsis: a systematic review.
Clin Infect Dis. 2004 Jul 1 ;39(1):38-46. Epub 2004 Jun 1 .

PubMed
Introducing students to the role of folk and popular health belief-systems in pa...
Introducing students to the role of folk and popular health belief-systems in patient care.
Acad Med. 1992 Sep ;67(9):566-8.

PubMed
Pediatric pedestrian trauma study: a pilot project.
Pediatric pedestrian trauma study: a pilot project.
Traffic Inj Prev. 2004 Jun ;5(2):132-6.

PubMed
Anaphylactoid reaction to iopamiro (after pretreatment).
Anaphylactoid reaction to iopamiro (after pretreatment).
Australas Radiol. 1992 May ;36(2):144-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: