FEBS Lett. 1992 Sep 14;309(3):394-8.

Mono ADP-ribosylation of transducin catalyzed by rod outer segment extract.

Ehret-Hilberer S, Nullans G, Aunis D, Virmaux N.

Source

INSERM Unité 338, Strasbourg, France.

Abstract

Transducin is the retinal rod outer segment (ROS)-specific G protein coupling the photoexcited rhodopsin to cyclic GMP-phosphodiesterase. The alpha subunit of transducin is known to be ADP-ribosylated by bacterial toxins. We investigated the possibility that transducin is modified in vitro by an endogenous ADP-ribosyltransferase activity. By using either ROS, cytosolic extract of ROS or purified transducin in the presence of [alpha-32P]nicotinamide adenine dinucleotide (NAD+), the alpha and beta subunits of transducin were found to be radiolabeled. The labeling was decreased by snake venom phosphodiesterase I (PDE I). The modification was shown to be mono ADP-ribosylation by analyses on thin layer chromatography of the PDE I-hydrolyzed products which revealed only 5'AMP residues. In addition we report that sodium nitroprusside activates the ADP-ribosylation of transducin.

PMID:: 1516716; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Functional modifications of transducin induced by cholera or pertussis-toxin-catalyzed ADP-ribosylation. [Eur J Biochem. 1992]
Functional modifications of transducin induced by cholera or pertussis-toxin-catalyzed ADP-ribosylation.
Bornancin F, Franco M, Bigay J, Chabre M. Eur J Biochem. 1992 Nov 15; 210(1):33-44.
Inhibition of the GTPase activity of transducin by an NAD+:arginine ADP-ribosyltransferase from turkey erythrocytes. [Biochem J. 1987]
Inhibition of the GTPase activity of transducin by an NAD+:arginine ADP-ribosyltransferase from turkey erythrocytes.
Watkins PA, Kanaho Y, Moss J. Biochem J. 1987 Dec 15; 248(3):749-54.
Suppression of GTP/T alpha-dependent activation of cGMP phosphodiesterase by ADP-ribosylation by its gamma subunit in amphibian rod photoreceptor membranes. [Biochemistry. 1999]
Suppression of GTP/T alpha-dependent activation of cGMP phosphodiesterase by ADP-ribosylation by its gamma subunit in amphibian rod photoreceptor membranes.
Bondarenko VA, Yamazaki M, Hayashi F, Yamazaki A. Biochemistry. 1999 Jun 15; 38(24):7755-63.
Characterization of transducin from bovine retinal rod outer segments. Mechanism and effects of cholera toxin-catalyzed ADP-ribosylation. [J Biol Chem. 1984]
Characterization of transducin from bovine retinal rod outer segments. Mechanism and effects of cholera toxin-catalyzed ADP-ribosylation.
Navon SE, Fung BK. J Biol Chem. 1984 May 25; 259(10):6686-93.
Review Visual transduction in rod outer segments. [Cell Signal. 1990]
Review Visual transduction in rod outer segments.
Takemoto DJ, Cunnick JM. Cell Signal. 1990; 2(2):99-104.

See reviews... See all...

Cited by 4 PubMed Central articles

Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism. [Mol Vis. 2007]
Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism.
Yang LP, Li Y, Zhu XA, Tso MO. Mol Vis. 2007 Jul 9; 13:1073-82. Epub 2007 Jul 9.
S-nitrosothiols modulate G protein-coupled receptor signaling in a reversible and highly receptor-specific manner. [BMC Cell Biol. 2005]
S-nitrosothiols modulate G protein-coupled receptor signaling in a reversible and highly receptor-specific manner.
Kokkola T, Savinainen JR, Mönkkönen KS, Retamal MD, Laitinen JT. BMC Cell Biol. 2005 Apr 25; 6(1):21. Epub 2005 Apr 25.
An ADP-ribosyltransferase as a potential target for nitric oxide action in hippocampal long-term potentiation. [Proc Natl Acad Sci U S A. 1994]
An ADP-ribosyltransferase as a potential target for nitric oxide action in hippocampal long-term potentiation.
Schuman EM, Meffert MK, Schulman H, Madison DV. Proc Natl Acad Sci U S A. 1994 Dec 6; 91(25):11958-62.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Mono ADP-ribosylation of transducin catalyzed by rod outer segment extract.
Mono ADP-ribosylation of transducin catalyzed by rod outer segment extract.
FEBS Lett. 1992 Sep 14 ;309(3):394-8.

PubMed
Adenoid cystic carcinoma of the head and neck: a review.
Adenoid cystic carcinoma of the head and neck: a review.
Curr Opin Otolaryngol Head Neck Surg. 2004 Apr ;12(2):127-32.

PubMed
Peptide motifs of closely related HLA class I molecules encompass substantial di...
Peptide motifs of closely related HLA class I molecules encompass substantial differences.
Eur J Immunol. 1992 Sep ;22(9):2453-6.

PubMed
Control of stochasticity in eukaryotic gene expression.
Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18 ;304(5678):1811-4. Epub 2004 May 27 .

PubMed
Accurate estimation of glomerular filtration rate in diabetic nephropathy from a...
Accurate estimation of glomerular filtration rate in diabetic nephropathy from age, body weight, and serum creatinine.
Diabetes Care. 1992 May ;15(5):609-12.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Mono ADP-ribosylation of transducin catalyzed by rod outer segment extract.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 4 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information