Biotechniques. 1992 May;12(5):650-4.

Rapid high resolution western blotting: from gel to image in a single day.

Source

Department of Cell Biology and Genetics, Sloan-Kettering Institute, New York, NY 10021.

Abstract

A streamlined protocol is described that allows high sensitivity antigen detection by Western blotting in a single day. The choice of membrane blotting matrix, as well as blocking reagents, has been optimized in order to allow rapid development of the blot with chemiluminescent reagents. The entire process, from gel to blot to a permanent, hard copy image on x-ray film, can be accomplished within six hours.

PMID:: 1515130; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Oncogene Protein pp60(v-src)

Grant Support

R01-CA52405/CA/NCI NIH HHS/United States

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