Plasmid. 1992 May;27(3):231-6.

Location of the origin of replication for the 7.5-kb Chlamydia trachomatis plasmid.

Tam JE, Davis CH, Thresher RJ, Wyrick PB.

Source

Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27514.

Abstract

The hypothetical origin of replication for the 7.5-kb plasmid common to Chlamydia trachomatis is believed to be in a region of the plasmid that contains four 22-bp tandem repeats preceded by an A-T-rich region. To test this hypothesis, replication of plasmid DNA in metabolically active reticulate bodies of the Lymphogranuloma venereum biovar of C. trachomatis was examined by electron microscopy. The results presented show that the origin of replication appears to be near the tandem repeats of pCHL2. In addition, replication of the 7.5-kb plasmid is unidirectional, and the copy number during replication is 7-10. The evidence presented suggests that C. trachomatis has a homologue to the Escherichia coli dnaA gene and that this homologue might be involved in replication of the C. trachomatis 7.5-kb plasmid.

PMID:: 1513879; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

DNA, Bacterial

Grant Support

AI 13446/AI/NIAID NIH HHS/United States

LinkOut - more resources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells. [Mol Microbiol. 1988]
The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells.
Comanducci M, Ricci S, Ratti G. Mol Microbiol. 1988 Jul; 2(4):531-8.
Characterization and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis. [Plasmid. 1987]
Characterization and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis.
Sriprakash KS, Macavoy ES. Plasmid. 1987 Nov; 18(3):205-14.
Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication. [Nucleic Acids Res. 1988]
Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication.
Hatt C, Ward ME, Clarke IN. Nucleic Acids Res. 1988 May 11; 16(9):4053-67.
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain. [BMC Genomics. 2009]
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain.
Seth-Smith HM, Harris SR, Persson K, Marsh P, Barron A, Bignell A, Bjartling C, Clark L, Cutcliffe LT, Lambden PR, et al. BMC Genomics. 2009 May 21; 10:239. Epub 2009 May 21.
The replication origin of the Methylomonas clara plasmid pBE-2. [DNA Seq. 1992]
The replication origin of the Methylomonas clara plasmid pBE-2.
Kües U, Stahl U. DNA Seq. 1992; 3(3):153-65.

See reviews... See all...

Cited by 8 PubMed Central articles

Review Recent advances in the understanding of Chlamydophila pecorum infections, sixteen years after it was named as the fourth species of the Chlamydiaceae family. [Vet Res. 2010]
Review Recent advances in the understanding of Chlamydophila pecorum infections, sixteen years after it was named as the fourth species of the Chlamydiaceae family.
Mohamad KY, Rodolakis A. Vet Res. 2010 May-Jun; 41(3):27. Epub 2009 Dec 10.
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain. [BMC Genomics. 2009]
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain.
Seth-Smith HM, Harris SR, Persson K, Marsh P, Barron A, Bignell A, Bjartling C, Clark L, Cutcliffe LT, Lambden PR, et al. BMC Genomics. 2009 May 21; 10:239. Epub 2009 May 21.
Chlamydia trachomatis load at matched anatomic sites: implications for screening strategies. [J Clin Microbiol. 2007]
Chlamydia trachomatis load at matched anatomic sites: implications for screening strategies.
Michel CE, Sonnex C, Carne CA, White JA, Magbanua JP, Nadala EC Jr, Lee HH. J Clin Microbiol. 2007 May; 45(5):1395-402. Epub 2007 Mar 21.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Location of the origin of replication for the 7.5-kb Chlamydia trachomatis plasm...
Location of the origin of replication for the 7.5-kb Chlamydia trachomatis plasmid.
Plasmid. 1992 May ;27(3):231-6.

PubMed
Cerebral toxocariasis: a possible cause of epileptic seizure in children.
Cerebral toxocariasis: a possible cause of epileptic seizure in children.
Childs Nerv Syst. 2004 Jul ;20(7):468-72. Epub 2004 May 12 .

PubMed
Infectious diseases and child day care.
Infectious diseases and child day care.
Pediatr Infect Dis J. 1992 Aug ;11(8 Suppl):S31-41.

PubMed
Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex...
Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism.
J Mol Biol. 2004 May 28 ;339(2):413-22.

PubMed
Neurologic improvement in pure motor hemiparesis: implications for clinical tria...
Neurologic improvement in pure motor hemiparesis: implications for clinical trials.
Neurology. 1992 Sep ;42(9):1713-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: