Electrophoresis. 1992 Jun;13(6):354-8.

The analysis of human serum transferrins with the PhastSystem: quantitation of microheterogeneity.

Source

Department of Chemical Pathology, Medical Faculty, Erasmus University Rotterdam, The Netherlands.

Abstract

The PhastSystem was used for three different analyses of human serum transferrins (Tf) by isoelectric focusing: (i) The distribution of iron over the two iron binding sites by separation of the four forms: apoTf, TfFeC, FeNTf and Fe2Tf by using less than 1 microL serum or plasma. The focused bands were visualized by immunoprecipitation and quantified by densitometry. (ii) The relative proportions of the diferic sialo-transferrin fractions in human sera or plasma were determined in a similar way. (iii) The presumed genetic variants can be determined. The methods are easy and reproducible and require much less sample (0.3 microL), time (1 h per run) and antibody (10 microL/sample) than crossed immunoelectrofocusing.

PMID:: 1505496; [PubMed - indexed for MEDLINE]

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Transferrin

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