Biotechniques. 1992 Jul;13(1):64-6.

Magnesium-dependent thermostability of DNase I.

Source

Department of Surgery, Oregon Health Sciences Univ., Portland.

Abstract

DNase I can be used to remove contaminating DNA from RNA samples. Heat treatment has been recommended as a method to inactivate DNase I enzymatic activity, thereby allowing subsequent reverse transcription and PCR amplification of DNase I treated RNA. We have found that inactivation of DNase I by heat treatment is strongly dependent on Mg2+ concentration. In addition, deoxyribonucleolytic activity of "inactivated" enzyme may be restored by changes in Mg2+ concentration following heat treatment. Caution should be exercised when using heat treatment alone as a method of DNase I inactivation.

PMID:: 1503777; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. [Biotechniques. 1996]
Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR.
Huang Z, Fasco MJ, Kaminsky LS. Biotechniques. 1996 Jun; 20(6):1012-4, 1016, 1018-20.
A novel mutation in the DNASE1 gene is related with protein instability and decreased enzyme activity in thyroid autoimmunity. [J Autoimmun. 2009]
A novel mutation in the DNASE1 gene is related with protein instability and decreased enzyme activity in thyroid autoimmunity.
Dittmar M, Bischofs C, Matheis N, Poppe R, Kahaly GJ. J Autoimmun. 2009 Feb; 32(1):7-13. Epub 2008 Nov 20.
Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion. [Biotechniques. 1997]
Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion.
Bauer P, Rolfs A, Regitz-Zagrosek V, Hildebrandt A, Fleck E. Biotechniques. 1997 Jun; 22(6):1128-32.
Review DNA recognition by DNase I. [J Mol Recognit. 1994]
Review DNA recognition by DNase I.
Suck D. J Mol Recognit. 1994 Jun; 7(2):65-70.
Review [High activity of deoxyribonuclease I identified in human pituitary gland--structure and function]. [Nihon Hoigaku Zasshi. 1998]
Review [High activity of deoxyribonuclease I identified in human pituitary gland--structure and function].
Yasuda T. Nihon Hoigaku Zasshi. 1998 Oct; 52(5):295-300.

See reviews... See all...

Cited by 3 PubMed Central articles

Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell. [BMC Biotechnol. 2005]
Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell.
Hartshorn C, Anshelevich A, Wangh LJ. BMC Biotechnol. 2005 Jan 13; 5:2. Epub 2005 Jan 13.
Molecular identification of bacteria by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene. [J Clin Microbiol. 1995]
Molecular identification of bacteria by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene.
Widjojoatmodjo MN, Fluit AC, Verhoef J. J Clin Microbiol. 1995 Oct; 33(10):2601-6.
Rapid identification of bacteria by PCR-single-strand conformation polymorphism. [J Clin Microbiol. 1994]
Rapid identification of bacteria by PCR-single-strand conformation polymorphism.
Widjojoatmodjo MN, Fluit AC, Verhoef J. J Clin Microbiol. 1994 Dec; 32(12):3002-7.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Magnesium-dependent thermostability of DNase I.
Magnesium-dependent thermostability of DNase I.
Biotechniques. 1992 Jul ;13(1):64-6.

PubMed
The yeast cell-cycle network is robustly designed.
The yeast cell-cycle network is robustly designed.
Proc Natl Acad Sci U S A. 2004 Apr 6 ;101(14):4781-6. Epub 2004 Mar 22 .

PubMed
The effects of repetitive community-wide ivermectin treatment on transmission of...
The effects of repetitive community-wide ivermectin treatment on transmission of Onchocerca volvulus in Guatemala.
Am J Trop Med Hyg. 1992 Aug ;47(2):170-80.

PubMed
A new clinical test for cervicocephalic kinesthetic sensibility: "the fly".
A new clinical test for cervicocephalic kinesthetic sensibility: "the fly".
Arch Phys Med Rehabil. 2004 Mar ;85(3):490-5.

PubMed
Serum 25-hydroxyvitamin D: distribution and determinants in the Swiss population...
Serum 25-hydroxyvitamin D: distribution and determinants in the Swiss population.
Am J Clin Nutr. 1992 Sep ;56(3):537-42.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Magnesium-dependent thermostability of DNase I.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Other Literature Sources

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 3 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information