Nature. 1992 Aug 13;358(6387):560-3.

A small metalloribozyme with a two-step mechanism.

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Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

Abstract

An RNA molecule consisting of an asymmetric internal loop of six nucleotides can be rapidly and specifically cleaved by Pb2+ in the presence of Mg2+. The 5' cleavage product terminates with a 3' phosphomonoester generated from a 2',3'-cyclic phosphodiester reaction intermediate. This two-step reaction mechanism resembles that of many protein ribonucleases but has not previously been observed for reactions catalysed by RNA.

PMID:: 1501711; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Solution Structure Of The Lead-Dependent Ribozyme, Nmr, Minimized Average Structure

PDB: 2LDZ

Source: synthetic construct

Method: Solution Nmr

See all 2 structures...

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A small metalloribozyme with a two-step mechanism.

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