Jpn J Pharmacol. 1992 Oct;60(2):79-84.

Inhibitory effects of hyaluronan on [14C]arachidonic acid release from labeled human synovial fibroblasts.

Tobetto K, Yasui T, Ando T, Hayaishi M, Motohashi N, Shinogi M, Mori I.

Source

Research Laboratories, Maruho Co., Ltd., Osaka, Japan.

Abstract

The effects of hyaluronan (HA) on the release of arachidonic acid (AA) from phospholipids induced by bradykinin in synovial fibroblasts of osteoarthritic patients were examined. HA inhibited [14C]AA release from prelabeled synovial cells stimulated with and without bradykinin 1 hr after incubation with HA and thereafter. The inhibitory effects of HA on [14C]AA release were dependent on the concentration and molecular weight of HA. However, inhibition of [14C]AA release by HA was not merely due to the viscosity of HA. The [14C]AA release induced by calcium-ionophore A23187 was also inhibited by HA with a high molecular weight. In addition, HA did not affect [14C]AA uptake by the cells. Our results suggest that HA with a high molecular weight elicits anti-inflammatory effects, at least in part, by inhibiting AA release in inflamed joints.

PMID:: 1479746; [PubMed - indexed for MEDLINE]

Free full text

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

The effects of a newly developed nonsteroidal anti-inflammatory drug (M-5011) on arachidonic acid metabolism in rheumatoid synovial fibroblasts. [Jpn J Pharmacol. 1997]
The effects of a newly developed nonsteroidal anti-inflammatory drug (M-5011) on arachidonic acid metabolism in rheumatoid synovial fibroblasts.
Tobetto K, Yamamoto Y, Kataoka M, Ando T, Sugimoto K, Himeno M. Jpn J Pharmacol. 1997 Dec; 75(4):371-9.
Arachidonic acid release from cultured human amnion cells: the effect of dexamethasone. [J Clin Endocrinol Metab. 1990]
Arachidonic acid release from cultured human amnion cells: the effect of dexamethasone.
Potestio FA, Olson DM. J Clin Endocrinol Metab. 1990 Mar; 70(3):647-54.
Calcium ionophore (A23187)- and arachidonic acid-stimulated prostaglandin release from microvascular endothelial cells: effects of calcium antagonists and calmodulin inhibitors. [J Pharmacol Exp Ther. 1987]
Calcium ionophore (A23187)- and arachidonic acid-stimulated prostaglandin release from microvascular endothelial cells: effects of calcium antagonists and calmodulin inhibitors.
Gerritsen ME, Nganele DM, Rodrigues AM. J Pharmacol Exp Ther. 1987 Mar; 240(3):837-46.
Review Potential mechanism of action of intra-articular hyaluronan therapy in osteoarthritis: are the effects molecular weight dependent? [Semin Arthritis Rheum. 2002]
Review Potential mechanism of action of intra-articular hyaluronan therapy in osteoarthritis: are the effects molecular weight dependent?
Ghosh P, Guidolin D. Semin Arthritis Rheum. 2002 Aug; 32(1):10-37.
Review The role of hyaluronic acid (hyaluronan) in health and disease: interactions with cells, cartilage and components of synovial fluid. [Clin Exp Rheumatol. 1994]
Review The role of hyaluronic acid (hyaluronan) in health and disease: interactions with cells, cartilage and components of synovial fluid.
Ghosh P. Clin Exp Rheumatol. 1994 Jan-Feb; 12(1):75-82.

See reviews... See all...

Cited by 4 PubMed Central articles

Managing joint pain in osteoarthritis: safety and efficacy of hylan G-F 20. [J Pain Res. 2009]
Managing joint pain in osteoarthritis: safety and efficacy of hylan G-F 20.
Conduah AH, Baker CL, Baker CL. J Pain Res. 2009 Jul 6; 2:87-98. Epub 2009 Jul 6.
The protective effect of sodium hyaluronate on the cartilage of rabbit osteoarthritis by inhibiting peroxisome proliferator-activated receptor-gamma messenger RNA expression. [Yonsei Med J. 2009]
The protective effect of sodium hyaluronate on the cartilage of rabbit osteoarthritis by inhibiting peroxisome proliferator-activated receptor-gamma messenger RNA expression.
Zhou JL, Liu SQ, Qiu B, Hu QJ, Ming JH, Peng H. Yonsei Med J. 2009 Dec 31; 50(6):832-7. Epub 2009 Dec 18.
Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction. [Arthritis Res Ther. 2004]
Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction.
Yoshida M, Sai S, Marumo K, Tanaka T, Itano N, Kimata K, Fujii K. Arthritis Res Ther. 2004; 6(6):R514-20. Epub 2004 Sep 22.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Inhibitory effects of hyaluronan on [14C]arachidonic acid release from labeled h...
Inhibitory effects of hyaluronan on [14C]arachidonic acid release from labeled human synovial fibroblasts.
Jpn J Pharmacol. 1992 Oct ;60(2):79-84.

PubMed
Effects of a high fat diet on the joints of aging mice.
Effects of a high fat diet on the joints of aging mice.
AMA Arch Pathol. 1950 Dec ;50(6):828-46.

PubMed
Association of the B-cell alloantigen DRw4 with rheumatoid arthritis.
Association of the B-cell alloantigen DRw4 with rheumatoid arthritis.
N Engl J Med. 1978 Apr 20 ;298(16):869-71.

PubMed
Up-regulation and altered distribution of lysyl oxidase in the central nervous s...
Up-regulation and altered distribution of lysyl oxidase in the central nervous system of mutant SOD1 transgenic mouse model of amyotrophic lateral sclerosis.
Brain Res Mol Brain Res. 2004 Jan 5 ;120(2):115-22.

PubMed
Quantitative scintigraphy of the sacroiliac joints.
Quantitative scintigraphy of the sacroiliac joints.
Clin Imaging. 1992 Oct-Dec ;16(4):230-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: