Biotechniques. 1992 Dec;13(6):901-5.

Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries.

Pinilla C, Appel JR, Blanc P, Houghten RA.

Source

Torrey Pines Institute for Molecular Studies, San Diego, CA 92121.

Abstract

We describe here a conceptually unique set of individual synthetic peptide combinatorial libraries (SPCLs), termed a positional scanning SPCL (PS-SPCL), that can be used for the rapid (i.e., a single day) identification of peptide sequences that bind with high affinity to antibodies, receptors or other acceptor molecules. The PS-SPCL described here is made up of six individual positional peptide libraries, each one consisting of hexamers with a single position defined and five positions as mixtures. As an example of the utility of such PS-SPCLs, the antigenic determinants recognized by two different monoclonal antibodies were correctly identified upon a single screening.

PMID:: 1476743; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Other Literature Sources

COS Scholar Universe

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands. [Life Sci. 1993]
The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands.
Dooley CT, Houghten RA. Life Sci. 1993; 52(18):1509-17.
Synthetic peptide combinatorial libraries (SPCLs): identification of the antigenic determinant of beta-endorphin recognized by monoclonal antibody 3E7. [Gene. 1993]
Synthetic peptide combinatorial libraries (SPCLs): identification of the antigenic determinant of beta-endorphin recognized by monoclonal antibody 3E7.
Pinilla C, Appel JR, Houghten RA. Gene. 1993 Jun 15; 128(1):71-6.
Two antipeptide monoclonal antibodies that recognize adhesive sequences in fibrinogen: identification of antigenic determinants and unrelated sequences using synthetic combinatorial libraries. [Biomed Pept Proteins Nucleic A...]
Two antipeptide monoclonal antibodies that recognize adhesive sequences in fibrinogen: identification of antigenic determinants and unrelated sequences using synthetic combinatorial libraries.
Pinilla C, Buencamino J, Appel JR, Houghten RA, Brassard JA, Ruggeri ZM. Biomed Pept Proteins Nucleic Acids. 1995; 1(3):199-204.
Review Random peptide libraries. [Curr Opin Biotechnol. 1994]
Review Random peptide libraries.
Scott JK, Craig L. Curr Opin Biotechnol. 1994 Feb; 5(1):40-8.
Review A review of the utility of soluble peptide combinatorial libraries. [Biopolymers. 1995]
Review A review of the utility of soluble peptide combinatorial libraries.
Pinilla C, Appel J, Blondelle S, Dooley C, Dörner B, Eichler J, Ostresh J, Houghten RA. Biopolymers. 1995; 37(3):221-40.

See reviews... See all...

Cited by 26 PubMed Central articles

Synthetic α-Conotoxin Mutants as Probes for Studying Nicotinic Acetylcholine Receptors and in the Development of Novel Drug Leads. [Toxins (Basel). 2010]
Synthetic α-Conotoxin Mutants as Probes for Studying Nicotinic Acetylcholine Receptors and in the Development of Novel Drug Leads.
Armishaw CJ. Toxins (Basel). 2010 Jun; 2(6):1471-99. Epub 2010 Jun 14.
A single T cell receptor bound to major histocompatibility complex class I and class II glycoproteins reveals switchable TCR conformers. [Immunity. 2011]
A single T cell receptor bound to major histocompatibility complex class I and class II glycoproteins reveals switchable TCR conformers.
Yin L, Huseby E, Scott-Browne J, Rubtsova K, Pinilla C, Crawford F, Marrack P, Dai S, Kappler JW. Immunity. 2011 Jul 22; 35(1):23-33. Epub 2011 Jun 16.
Peptide binding predictions for HLA DR, DP and DQ molecules. [BMC Bioinformatics. 2010]
Peptide binding predictions for HLA DR, DP and DQ molecules.
Wang P, Sidney J, Kim Y, Sette A, Lund O, Nielsen M, Peters B. BMC Bioinformatics. 2010 Nov 22; 11:568. Epub 2010 Nov 22.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Rapid identification of high affinity peptide ligands using positional scanning ...
Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries.
Biotechniques. 1992 Dec ;13(6):901-5.

PubMed
Relationships of perceived benefits and barriers to physical activity, physical ...
Relationships of perceived benefits and barriers to physical activity, physical activity participation and physical fitness in Hong Kong female adolescents.
J Sports Med Phys Fitness. 2003 Dec ;43(4):523-9.

PubMed
Myelinated nerve fiber supply and muscle spindles in the respiratory muscles of ...
Myelinated nerve fiber supply and muscle spindles in the respiratory muscles of cat: quantitative study.
Anat Embryol (Berl). 1978 Feb 20 ;152(2):171-92.

PubMed
Quality of life and symptom severity for individuals with chronic fatigue syndro...
Quality of life and symptom severity for individuals with chronic fatigue syndrome: findings from a randomized clinical trial.
Am J Occup Ther. 2004 Jan-Feb ;58(1):35-43.

PubMed
Re: "Defining exposure in case-control studies: a new approach".
Re: "Defining exposure in case-control studies: a new approach".
Am J Epidemiol. 1992 Nov 15 ;136(10):1294.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: