Yi Chuan Xue Bao. 1992;19(4):362-8.

[Isolation of vitamin B2 auxotroph and preliminary genetic mapping in Salmonella typhimurium].

[Article in Chinese]

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Institute of Microbiology, Academia Sinica, Beijing.

Abstract

The first independent vitamin B2 auxotrophs of Salmonella typhimurium were obtained by using selective medium containing extraordinarily high concentration of B2 (300 micrograms/ml) after mutagenesis. Transduction analysis showed that 21 B2 auxotrophs could be divided into four groups, which were unlinked to each other. It means that at least 4 structural genes were involved in B2 biosynthetic pathway. Preliminary genetic mapping indicated that 2 genes located in 7'-22' and other 2 located in 22'-34' and 61.5'-69' region on the genetic map of S. typhimurium respectively.

PMID:: 1466913; [PubMed - indexed for MEDLINE]

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