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    Science. 2004 Jan 23;303(5657):521-3. Epub 2003 Nov 20.

    One-way control of FWA imprinting in Arabidopsis endosperm by DNA methylation.

    Source

    Integrated Genetics, National Institute of Genetics, Mishima 411-8540, Japan. tekinosh@lab.nig.ac.jp

    Abstract

    The Arabidopsis FWA gene was initially identified from late-flowering epigenetic mutants that show ectopic FWA expression associated with heritable hypomethylation of repeats around transcription starting sites. Here, we show that wild-type FWA displays imprinted (maternal origin-specific) expression in endosperm. The FWA imprint depends on the maintenance DNA methyltransferase MET1, as is the case in mammals. Unlike mammals, however, the FWA imprint is not established by allele-specific de novo methylation. It is established by maternal gametophyte-specific gene activation, which depends on a DNA glycosylase gene, DEMETER. Because endosperm does not contribute to the next generation, the activated FWA gene need not be silenced again. Double fertilization enables plants to use such "one-way" control of imprinting and DNA methylation in endosperm.

    PMID:
    14631047
    [PubMed - indexed for MEDLINE]

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