Int J Radiat Biol. 1992 Dec;62(6):673-8.

Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.

Schmid E, Zitzelsberger H, Braselmann H, Gray JW, Bauchinger M.

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Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.

Abstract

Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

PMID:: 1362760; [PubMed - indexed for MEDLINE]

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