PCR Methods Appl. 1992 Feb;1(3):160-3.

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

Singer-Sam J, LeBon JM, Dai A, Riggs AD.

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Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.

Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

PMID:: 1282066; [PubMed - indexed for MEDLINE]

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R01 AG08196/AG/NIA NIH HHS/United States

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Parental imprinting studied by allele-specific primer extension after PCR: paternal X chromosome-linked genes are transcribed prior to preferential paternal X chromosome inactivation. [Proc Natl Acad Sci U S A. 1992]
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A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

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