Ann Trop Med Parasitol. 2003 Mar;97(2):131-7.

Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present.

Padley D, Moody AH, Chiodini PL, Saldanha J.

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Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms EN6 3QG, UK. dpadley@nibsc.ac.uk

Abstract

A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.

PMID:: 12803868; [PubMed - indexed for MEDLINE]

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DNA, Protozoan

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Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present.

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