Appl Environ Microbiol. 1992 Oct;58(10):3417-8.

Differential amplification of rRNA genes by polymerase chain reaction.

Reysenbach AL, Giver LJ, Wickham GS, Pace NR.

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Department of Biology, Indiana University, Bloomington 47405.

Abstract

The polymerase chain reaction (PCR) is used widely to recover rRNA genes from naturally occurring communities for analysis of population constituents. We have found that this method can result in differential amplification of different rRNA genes. In particular, rDNAs of extremely thermophilic archaebacteria often cannot be amplified by the usual PCR methods. The addition of 5% (wt/vol) acetamide to a PCR mixture containing both archaebacterial and yeast DNA templates minimized nonspecific annealing of the primers and prevented preferential amplification of the yeast small-subunit rRNA genes.

PMID:: 1280061; [PubMed - indexed for MEDLINE]
PMCID:: PMC183115

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Differential amplification of rRNA genes by polymerase chain reaction.

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