J Biomed Sci. 2002 Nov-Dec;9(6 Pt 2):721-6.

A macrophage-tropic HIV-1 that expresses green fluorescent protein and infects alveolar and blood monocyte-derived macrophages.

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Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.

Abstract

We describe the construction of a macrophage-tropic HIV-1 molecular clone, pNLAD8-EGFP, which expresses enhanced green fluorescent protein. We show that NLAD8-EGFP can infect monocyte-derived macrophages as well as alveolar macrophages. NLAD8-EGFP-infected macrophages can be easily and sensitively detected based on the visualization of intracellular green fluorescent protein.

PMID:: 12432239; [PubMed - indexed for MEDLINE]

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