Nat Rev Genet. 2002 Oct;3(10):737-47.

Gene silencing in mammals by small interfering RNAs.

Source

Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17-526, Cambridge, Massachusetts 02139, USA. mmcmanus@mit.edu

Abstract

Among the 3 billion base pairs of the human genome, there are approximately 30,000-40,000 protein-coding genes, but the function of at least half of them remains unknown. A new tool - short interfering RNAs (siRNAs) - has now been developed for systematically deciphering the functions and interactions of these thousands of genes. siRNAs are an intermediate of RNA interference, the process by which double-stranded RNA silences homologous genes. Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology.

PMID:: 12360232; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Research Materials

Dicer antibody [13D6] - ChIP Grade - Abcam plc

Libraries

LinkOut Holdings

Supplemental Content

Related citations

Review Targeted gene silencing by small interfering RNA-based knock-down technology. [Curr Pharm Biotechnol. 2004]
Review Targeted gene silencing by small interfering RNA-based knock-down technology.
Zhang J, Hua ZC. Curr Pharm Biotechnol. 2004 Feb; 5(1):1-7.
Review [RNA interference and its possible use in cancer therapy]. [Bull Cancer. 2004]
Review [RNA interference and its possible use in cancer therapy].
Ait-Si-Ali S, Guasconi V, Harel-Bellan A. Bull Cancer. 2004 Jan; 91(1):15-8.
Review The role of small RNAs in human diseases: potential troublemaker and therapeutic tools. [Med Res Rev. 2005]
Review The role of small RNAs in human diseases: potential troublemaker and therapeutic tools.
Gong H, Liu CM, Liu DP, Liang CC. Med Res Rev. 2005 May; 25(3):361-81.
Review [Role of siRNAS and miRNAS in the processes of RNA-mediated gene silencing during viral infections]. [Tsitol Genet. 2009]
Review [Role of siRNAS and miRNAS in the processes of RNA-mediated gene silencing during viral infections].
Krul'ko IV, Ust'ianenko DO, Polishchuk VP. Tsitol Genet. 2009 Jan-Feb; 43(1):78-90.
Review [RNA binding proteins in the RNA interference phenomenon]. [Mol Biol (Mosk). 2006]
Review [RNA binding proteins in the RNA interference phenomenon].
Kotel'nikov RN, Shpiz SG, Kalmykova AI, Gvozdev VA. Mol Biol (Mosk). 2006 Jul-Aug; 40(4):595-608.

See reviews... See all...

Cited by over 100 PubMed Central articles

Lentiviral Transgenic MicroRNA-Based shRNA Suppressed Mouse Cytochromosome P450 3A (CYP3A) Expression in a Dose-Dependent and Inheritable Manner. [PLoS One. 2012]
Lentiviral Transgenic MicroRNA-Based shRNA Suppressed Mouse Cytochromosome P450 3A (CYP3A) Expression in a Dose-Dependent and Inheritable Manner.
Wang Y, Hu HH, Pang H, Zhou XY, Yu LS, Wang LL, Liu C, Guo KN, Zhao C, Liu Q, et al. PLoS One. 2012; 7(1):e30560. Epub 2012 Jan 24.
Inhibition of dengue virus entry and multiplication into monocytes using RNA interference. [PLoS Negl Trop Dis. 2011]
Inhibition of dengue virus entry and multiplication into monocytes using RNA interference.
Alhoot MA, Wang SM, Sekaran SD. PLoS Negl Trop Dis. 2011 Nov; 5(11):e1410. Epub 2011 Nov 29.
siPRED: predicting siRNA efficacy using various characteristic methods. [PLoS One. 2011]
siPRED: predicting siRNA efficacy using various characteristic methods.
Pan WJ, Chen CW, Chu YW. PLoS One. 2011; 6(11):e27602. Epub 2011 Nov 10.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Gene silencing in mammals by small interfering RNAs.
Gene silencing in mammals by small interfering RNAs.
Nat Rev Genet. 2002 Oct ;3(10):737-47.

PubMed
Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replicat...
Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue.
Retrovirology. 2010 Jan 15 ;7:1.

PubMed
Identification and characterization of human cytomegalovirus-encoded microRNAs.
Identification and characterization of human cytomegalovirus-encoded microRNAs.
J Virol. 2005 Sep ;79(18):12095-9.

PubMed
WHO admits its target on AIDS drugs may be unrealistic.
WHO admits its target on AIDS drugs may be unrealistic.
BMJ. 2004 May 15 ;328(7449):1151.

PubMed
Design of HIV vectors for efficient gene delivery into human hematopoietic cells...
Design of HIV vectors for efficient gene delivery into human hematopoietic cells.
Mol Ther. 2002 Apr ;5(4):479-84.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: