Science. 2001 Aug 3;293(5531):834-8. Epub 2001 Jul 12.

A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.

Hutvágner G, McLachlan J, Pasquinelli AE, Bálint E, Tuschl T, Zamore PD.

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Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA.

Abstract

The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

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Science. 2001 Aug 3;293(5531):811-3.

PMID:: 11452083; [PubMed - indexed for MEDLINE]

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A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.

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