Distributions of the MAP values for the most probable motifs from the study set (183 data sets) and the full set (2097 data sets). (A) The distribution of MAP values for the full set compared to the study set, illustrating the shift to the left (toward lower MAPs) for the full set (see text) and indicating the relative number in the study set of genes that have experimentally identified sites compared to the full set. (B) The distribution of MAP values for the study set broken down according to the number of orthologs detected for each gene. (C) The distribution of MAP values for the full set broken down according to the number of orthologs detected for each gene. Comparison of (B) and (C) again illustrates the shift toward lower MAP values for the full set compared to the study set, as well as the observation that when data were available from only two species the predictions typically had lower MAP values.

SDS–PAGE gel showing affinity purification of YijC. Escherichia coli MG1655 extracts, passed over a purH pre-column, were fractionated on DNA affinity columns carrying sequences predicted to be TF-binding sites upstream of the fabA, fabB or yqfA genes or a control column carrying a known FadR site upstream of fadB (see Materials and Methods). A silver stained SDS–PAGE gel of representative fractions eluted from the columns with 0.8 M NaCl is shown. M, molecular weight markers; lane 1, fabA column; lane 2, fabB column; lane 3, yqfA column; lane 4, fadB column. Mass spectrometry analysis identified the 26 kDa protein bound specifically to the fabA, fabB and yqfA columns as YijC and the protein bound to the fadB column as FadR.

Snapshot from the fabA Gene Page on our web site illustrating the data available. (A) At the top of each Gene Page are given the gene name as it appears in both the E.coli genome GenBank entry (27) and in EcoGene (28; http://bmb.med.miami.edu/EcoGene/EcoWeb/index.html), as is the name of the divergently transcribed gene when one exists. The species in which orthologs were detected for the gene are indicated (EC, E.coli; ST, Salmonella typhi; YP, Yersinia pestis; HI, Haemophilus influenzae; AA, Actinobacillus actinomycetemcomitans; VC, Vibrio cholerae; SP, Shewanella putrefaciens; PA, Pseudomonas aeruginosa; TF, Thiobacillus ferrooxidans). For those genes with a documented regulatory site(s), the reference(s), the genomic coordinates of the site(s) and the site type(s) are given. Information from up to three predictions (ordered by MAP value) are then described. For each prediction the species in which a site was predicted are indicated, as are the total number of sites and the number of sites in the E.coli data, followed by the MAP value of the motif. Links are provided to the motif model (B), represented as a sequence logo (29), and to two representations of the sites that were identified: a sequence logo (C) and a sequence alignment with site probabilities (D). The E.coli genomic coordinates of the site (an R indicates that the solution sequence given is the reverse complement of that in the GenBank entry), as well as the site sequence plus 5 flanking bp, are given. When a predicted site overlaps a previously documented site the site type (TF name or stem–loop) is indicated. If a predicted site overlaps an E.coli intergenic repeat (28), that is also reported. While analysis of the study set for correlation to documented TF-binding sites was confined to the most probable motif predictions, up to three predictions (ordered by MAP value) are described on our web site for each gene, since many genes are regulated by more than one transcription factor. The most probable motif (the YijC-binding site) detected in the fabA data is shown.