Science. 2000 Nov 17;290(5495):1368-72.

Intracellular parasitism by Histoplasma capsulatum: fungal virulence and calcium dependence.

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Department of Molecular Microbiology, Campus Box 8230, Washington University School of Medicine, St. Louis, MO 63110, USA.

Abstract

Histoplasma capsulatum is an effective intracellular parasite of macrophages and causes the most prevalent fungal respiratory disease in the United States. A "dimorphic" fungus, H. capsulatum exists as a saprophytic mold in soil and converts to the parasitic yeast form after inhalation. Only the yeasts secrete a calcium-binding protein (CBP) and can grow in calcium-limiting conditions. To probe the relation between calcium limitation and intracellular parasitism, we designed a strategy to disrupt CBP1 in H. capsulatum using a telomeric linear plasmid and a two-step genetic selection. The resultingcbp1 yeasts no longer grew when deprived of calcium, and they were also unable to destroy macrophages in vitro or proliferate in a mouse model of pulmonary infection.

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Science. 2000 Nov 17;290(5495):1311-2.

PMID:: 11082066; [PubMed - indexed for MEDLINE]

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Intracellular parasitism by Histoplasma capsulatum: fungal virulence and calcium dependence.

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