Science. 2000 Nov 10;290(5494):1159-62.

Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase.

Wu-Scharf D, Jeong B, Zhang C, Cerutti H.

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School of Biological Sciences and Plant Science Initiative, University of Nebraska-Lincoln, E211 Beadle Center, Post Office Box 880666, Lincoln, NE 68588, USA.

Abstract

The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.

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Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase.

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