Biotechniques. 2000 Sep;29(3):548-50, 552-4, 556 passim.

A concise guide to cDNA microarray analysis.

Hegde P, Qi R, Abernathy K, Gay C, Dharap S, Gaspard R, Hughes JE, Snesrud E, Lee N, Quackenbush J.

Source

Institute for Genomic Research, Rockville, MD, USA.

Abstract

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

PMID:: 10997270; [PubMed - indexed for MEDLINE]

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R01 CA77049-01/CA/NCI NIH HHS/United States

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