Science. 2000 Jun 23;288(5474):2215-9.

Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

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CREST Research Project, Japan Science and Technology Corporation, Kyoto, Japan.

Abstract

Mammalian kinetochores contain the centromere-specific histone H3 variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G1-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.

PMID:: 10864871; [PubMed - indexed for MEDLINE]

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Related citations

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Cited by over 100 PubMed Central articles

Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres. [J Biol Chem. 2011]
Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres.
Choi ES, Strålfors A, Castillo AG, Durand-Dubief M, Ekwall K, Allshire RC. J Biol Chem. 2011 Jul 1; 286(26):23600-7. Epub 2011 Apr 28.
Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast. [PLoS One. 2011]
Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.
Shiroiwa Y, Hayashi T, Fujita Y, Villar-Briones A, Ikai N, Takeda K, Ebe M, Yanagida M. PLoS One. 2011 Mar 21; 6(3):e17761. Epub 2011 Mar 21.
The FUN30 chromatin remodeler, Fft3, protects centromeric and subtelomeric domains from euchromatin formation. [PLoS Genet. 2011]
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